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A novel genetic strategy reveals unexpected roles of the Swi-Snf-like chromatin-remodeling BAF complex in thymocyte development | |
2008 | |
发表期刊 | JOURNAL OF EXPERIMENTAL MEDICINE |
ISSN | 0022-1007 |
EISSN | 1540-9538 |
卷号 | 205期号:12页码:2813-2825 |
发表状态 | 已发表 |
DOI | 10.1084/jem.20080938 |
摘要 | We have developed a general strategy for creating littermates bearing either a tissue-specific point mutation or deletion in any target gene, and used the method to dissect the roles of Brg, the ATPase subunit of the chromatin-remodeling Brg-associated factor (BAF) complex, in early thymocyte development. We found that a point mutation that inactivates the Brg ATPase recapitulates multiple defects previously described for Brg deletion (Chi, T. H., M. Wan, P. P. Lee, K. Akashi, D. Metzger, P. Chambon, C. B. Wilson, and G. R. Crabtree. 2003. Immunity. 19: 169-182). However, the point mutant helps reveal unexpected roles of Brg in CD25 repression and CD4 activation. Surprisingly, CD4 activation occurs independently of the Brg ATPase and is perhaps mediated by physical interactions between Brg and the CD4 locus. Our study thus suggests that the BAF complex harbors novel activities that can be necessary and even sufficient for stimulating transcription from an endogenous chromatin template in the absence of Brg-dependent remodeling of that template. We conclude that conditional point mutants, rarely used in mammalian genetics, can help uncover important gene functions undetectable or overlooked in deletion mutants. |
URL | 查看原文 |
收录类别 | SCI |
WOS研究方向 | Immunology ; Research & Experimental Medicine |
WOS类目 | Immunology ; Medicine, Research & Experimental |
WOS记录号 | WOS:000261295300015 |
WOS关键词 | T-CELL DEVELOPMENT ; MAMMALIAN SWI/SNF COMPLEXES ; CD4 GENE ; EXPRESSION ; TRANSCRIPTION ; SELECTION ; BRG1 ; MECHANISMS ; PROMOTER ; LINEAGE |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/120299 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Chi, Tian |
作者单位 | 1.Yale Univ, Sch Med, Dept Immunobiol, New Haven, CT 06520 USA 2.NHLBI, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA |
推荐引用方式 GB/T 7714 | Jani, Anant,Wan, Mimi,Zhang, Jianmin,et al. A novel genetic strategy reveals unexpected roles of the Swi-Snf-like chromatin-remodeling BAF complex in thymocyte development[J]. JOURNAL OF EXPERIMENTAL MEDICINE,2008,205(12):2813-2825. |
APA | Jani, Anant.,Wan, Mimi.,Zhang, Jianmin.,Cui, Kairong.,Wu, Jie.,...&Chi, Tian.(2008).A novel genetic strategy reveals unexpected roles of the Swi-Snf-like chromatin-remodeling BAF complex in thymocyte development.JOURNAL OF EXPERIMENTAL MEDICINE,205(12),2813-2825. |
MLA | Jani, Anant,et al."A novel genetic strategy reveals unexpected roles of the Swi-Snf-like chromatin-remodeling BAF complex in thymocyte development".JOURNAL OF EXPERIMENTAL MEDICINE 205.12(2008):2813-2825. |
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