Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors
2013
发表期刊PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (IF:9.4[JCR-2023],10.8[5-Year])
ISSN0027-8424
卷号110期号:52页码:21048-21053
发表状态已发表
DOI10.1073/pnas.1319097110
摘要

We describe a transcription activator-like effector (TALE)-based strategy, termed "TALEColor," for labeling specific repetitive DNA sequences in human chromosomes. We designed TALEs for the human telomeric repeat and fused them with any of numerous fluorescent proteins (FPs). Expression of these TALE-telomere-FP fusion proteins in human osteosarcoma's (U2OS) cells resulted in bright signals coincident with telomeres. We also designed TALEs for centromeric sequences unique to certain chromosomes, enabling us to localize specific human chromosomes in live cells. Meanwhile we generated TALE-FPs in vitro and used them as probes to detect telomeres in fixed cells. Using human cells with different average telomere lengths, we found that the TALEColor signals correlated positively with telomere length. In addition, suspension cells were followed by imaging flow cytometry to resolve cell populations with differing telomere lengths. These methods may have significant potential both for basic chromosome and genome research as well as in clinical applications.

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收录类别SCI
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000328858800052
WOS关键词ALPHA-SATELLITE DNA ; HUMAN-CELLS ; TELOMERASE ACTIVITY ; DYNAMICS ; PROTEINS
原始文献类型Article
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文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/120137
专题个人在本单位外知识产出
通讯作者Ma, Hanhui; Pederson, Thoru
作者单位
Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Program Cell & Dev Dynam, Worcester, MA 01605 USA
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Ma, Hanhui,Reyes-Gutierrez, Pablo,Pederson, Thoru. Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(52):21048-21053.
APA Ma, Hanhui,Reyes-Gutierrez, Pablo,&Pederson, Thoru.(2013).Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,110(52),21048-21053.
MLA Ma, Hanhui,et al."Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.52(2013):21048-21053.
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