ShanghaiTech University Knowledge Management System
Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors | |
2013 | |
发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (IF:9.4[JCR-2023],10.8[5-Year]) |
ISSN | 0027-8424 |
卷号 | 110期号:52页码:21048-21053 |
发表状态 | 已发表 |
DOI | 10.1073/pnas.1319097110 |
摘要 | We describe a transcription activator-like effector (TALE)-based strategy, termed "TALEColor," for labeling specific repetitive DNA sequences in human chromosomes. We designed TALEs for the human telomeric repeat and fused them with any of numerous fluorescent proteins (FPs). Expression of these TALE-telomere-FP fusion proteins in human osteosarcoma's (U2OS) cells resulted in bright signals coincident with telomeres. We also designed TALEs for centromeric sequences unique to certain chromosomes, enabling us to localize specific human chromosomes in live cells. Meanwhile we generated TALE-FPs in vitro and used them as probes to detect telomeres in fixed cells. Using human cells with different average telomere lengths, we found that the TALEColor signals correlated positively with telomere length. In addition, suspension cells were followed by imaging flow cytometry to resolve cell populations with differing telomere lengths. These methods may have significant potential both for basic chromosome and genome research as well as in clinical applications. |
URL | 查看原文 |
收录类别 | SCI |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000328858800052 |
WOS关键词 | ALPHA-SATELLITE DNA ; HUMAN-CELLS ; TELOMERASE ACTIVITY ; DYNAMICS ; PROTEINS |
原始文献类型 | Article |
引用统计 | 正在获取...
|
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/120137 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Ma, Hanhui; Pederson, Thoru |
作者单位 | Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Program Cell & Dev Dynam, Worcester, MA 01605 USA |
推荐引用方式 GB/T 7714 | Ma, Hanhui,Reyes-Gutierrez, Pablo,Pederson, Thoru. Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(52):21048-21053. |
APA | Ma, Hanhui,Reyes-Gutierrez, Pablo,&Pederson, Thoru.(2013).Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,110(52),21048-21053. |
MLA | Ma, Hanhui,et al."Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.52(2013):21048-21053. |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 |
修改评论
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。