Targeting of Fzr/Cdh1 for timely activation of the APC/C at the centrosome during mitotic exit

doi:10.1038/ncomms12607

	Targeting of Fzr/Cdh1 for timely activation of the APC/C at the centrosome during mitotic exit
	Meghini, Francesco ; Martins, Torcato ; Tait, Xavier ; Fujimitsu, Kazuyuki ; Yamano, Hiroyuki ; Glover, David M.; Kimata, Yuu
	2016
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
ISSN	2041-1723
卷号	7
发表状态	已发表
DOI	10.1038/ncomms12607
摘要	A multi-subunit ubiquitin ligase, the anaphase-promoting complex/cyclosome (APC/C), regulates critical cellular processes including the cell cycle. To accomplish its diverse functions, APC/C activity must be precisely regulated in time and space. The interphase APC/C activator Fizzy-related (Fzr or Cdh1) is localized at centrosomes in animal cells. However, neither the mechanism of its localization nor its importance is clear. Here we identify the centrosome component Spd2 as a major partner of Fzr in Drosophila. The localization of Fzr to the centriole during interphase depends on direct interaction with Spd2. By generating Spd2 mutants unable to bind Fzr, we show that centrosomal localization of Fzr is essential for optimal APC/C activation towards its centrosomal substrate Aurora A. Finally, we show that Spd2 is also a novel APC/CFzr substrate. Our study is the first to demonstrate the critical importance of distinct subcellular pools of APC/C activators in the spatiotemporal control of APC/C activity.
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收录类别	SCI
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000383663300001
WOS关键词	ANAPHASE-PROMOTING COMPLEX/CYCLOSOME ; AURORA-A KINASE ; PERICENTRIOLAR MATERIAL ; CENTRIOLE DUPLICATION ; CELL-CYCLE ; DROSOPHILA NEUROBLASTS ; PROTEIN COMPLEXES ; SELF-RENEWAL ; MITOSIS ; SPINDLE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/119996
专题	个人在本单位外知识产出
通讯作者	Kimata, Yuu
作者单位	1.Univ Cambridge, Dept Genet, Cell Cycle Dev Grp, Downing St, Cambridge CB2 3EH, England 2.Univ Cambridge, Dept Genet, Cell Cycle Genet Grp, Downing St, Cambridge CB2 3EH, England 3.UCL, UCL Canc Inst, Cell Cycle Control Grp, 72 Huntley St, London WC1E 6DD, England
推荐引用方式 GB/T 7714	Meghini, Francesco,Martins, Torcato,Tait, Xavier,et al. Targeting of Fzr/Cdh1 for timely activation of the APC/C at the centrosome during mitotic exit[J]. NATURE COMMUNICATIONS,2016,7.
APA	Meghini, Francesco.,Martins, Torcato.,Tait, Xavier.,Fujimitsu, Kazuyuki.,Yamano, Hiroyuki.,...&Kimata, Yuu.(2016).Targeting of Fzr/Cdh1 for timely activation of the APC/C at the centrosome during mitotic exit.NATURE COMMUNICATIONS,7.
MLA	Meghini, Francesco,et al."Targeting of Fzr/Cdh1 for timely activation of the APC/C at the centrosome during mitotic exit".NATURE COMMUNICATIONS 7(2016).