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Modulation of beta-Catenin Signaling by Glucagon Receptor Activation | |
2012 | |
发表期刊 | PLOS ONE |
ISSN | 1932-6203 |
卷号 | 7期号:3 |
发表状态 | 已发表 |
DOI | 10.1371/journal.pone.0033676 |
摘要 | The glucagon receptor (GCGR) is a member of the class B G protein-coupled receptor family. Activation of GCGR by glucagon leads to increased glucose production by the liver. Thus, glucagon is a key component of glucose homeostasis by counteracting the effect of insulin. In this report, we found that in addition to activation of the classic cAMP/protein kinase A (PKA) pathway, activation of GCGR also induced beta-catenin stabilization and activated beta-catenin-mediated transcription. Activation of beta-catenin signaling was PKA-dependent, consistent with previous reports on the parathyroid hormone receptor type 1 (PTH1R) and glucagon-like peptide 1 (GLP-1R) receptors. Since low-density-lipoprotein receptor-related protein 5 (Lrp5) is an essential co-receptor required for Wnt protein mediated beta-catenin signaling, we examined the role of Lrp5 in glucagon-induced beta-catenin signaling. Cotransfection with Lrp5 enhanced the glucagon-induced beta-catenin stabilization and TCF promoter-mediated transcription. Inhibiting Lrp5/6 function using Dickkopf-1(DKK1) or by expression of the Lrp5 extracellular domain blocked glucagon-induced beta-catenin signaling. Furthermore, we showed that Lrp5 physically interacted with GCGR by immunoprecipitation and bioluminescence resonance energy transfer assays. Together, these results reveal an unexpected crosstalk between glucagon and beta-catenin signaling, and may help to explain the metabolic phenotypes of Lrp5/6 mutations. |
URL | 查看原文 |
收录类别 | SCI |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000303309100085 |
出版者 | PUBLIC LIBRARY SCIENCE |
WOS关键词 | PROTEIN-COUPLED RECEPTORS ; PARATHYROID-HORMONE ; CELL-GROWTH ; WNT ; PATHWAY ; METABOLISM ; MECHANISMS ; MEMBRANE ; DISEASE ; FAMILY |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/119807 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Ke, Jiyuan; Xu, H. Eric |
作者单位 | 1.Van Andel Res Inst, Lab Struct Sci, Grand Rapids, MI 49503 USA 2.Van Andel Res Inst, Lab Cell Signaling & Carcinogenesis, Grand Rapids, MI USA 3.Chinese Acad Sci, Shanghai Inst Mat Med, VARI SIMM Ctr, Ctr Struct & Funct Drug Targets, Shanghai 200031, Peoples R China 4.Mayo Clin Scottsdale, Dept Mol Pharmacol & Expt Therapeut, Scottsdale, AZ USA |
推荐引用方式 GB/T 7714 | Ke, Jiyuan,Zhang, Chenghai,Harikumar, Kaleeckal G.,et al. Modulation of beta-Catenin Signaling by Glucagon Receptor Activation[J]. PLOS ONE,2012,7(3). |
APA | Ke, Jiyuan.,Zhang, Chenghai.,Harikumar, Kaleeckal G..,Zylstra-Diegel, Cassandra R..,Wang, Liren.,...&Xu, H. Eric.(2012).Modulation of beta-Catenin Signaling by Glucagon Receptor Activation.PLOS ONE,7(3). |
MLA | Ke, Jiyuan,et al."Modulation of beta-Catenin Signaling by Glucagon Receptor Activation".PLOS ONE 7.3(2012). |
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