ShanghaiTech University Knowledge Management System
Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase | |
2011 | |
发表期刊 | NUCLEIC ACIDS RESEARCH |
ISSN | 0305-1048 |
EISSN | 1362-4962 |
卷号 | 39期号:1页码:235-247 |
发表状态 | 已发表 |
DOI | 10.1093/nar/gkq763 |
摘要 | To prevent potential errors in protein synthesis, some aminoacyl-transfer RNA (tRNA) synthetases have evolved editing mechanisms to hydrolyze misactivated amino acids (pre-transfer editing) or misacylated tRNAs (post-transfer editing). Class Ia leucyl-tRNA synthetase (LeuRS) may misactivate various natural and non-protein amino acids and then mischarge tRNA(Leu). It is known that the fidelity of prokaryotic LeuRS depends on multiple editing pathways to clear the incorrect intermediates and products in the every step of aminoacylation reaction. Here, we obtained human cytoplasmic LeuRS (hcLeuRS) and tRNA(Leu) (hctRNA(Leu)) with high activity from Escherichia coli overproducing strains to study the synthetic and editing properties of the enzyme. We revealed that hcLeuRS could adjust its editing strategy against different non-cognate amino acids. HcLeuRS edits norvaline predominantly by post-transfer editing; however, it uses mainly pre-transfer editing to edit a-amino butyrate, although both amino acids can be charged to tRNA(Leu). Post-transfer editing as a final checkpoint of the reaction was very important to prevent mis-incorporation in vitro. These results provide insight into the modular editing pathways created to prevent genetic code ambiguity by evolution. |
URL | 查看原文 |
收录类别 | SCI |
WOS研究方向 | Biochemistry & Molecular Biology |
WOS类目 | Biochemistry & Molecular Biology |
WOS记录号 | WOS:000286008500024 |
出版者 | OXFORD UNIV PRESS |
WOS关键词 | I TRANSFER-RNA ; GENETIC-CODE AMBIGUITY ; ESCHERICHIA-COLI ; CELL VIABILITY ; PROTEIN-SYNTHESIS ; STRUCTURAL BASIS ; COMPLEX ; AMINOACYLATION ; DISCRIMINATION ; SPECIFICITY |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/119759 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Wang, En-Duo |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol,Grad Sch, Shanghai 200031, Peoples R China 2.Univ Strasbourg, CNRS, Inst Biol Mol & Cellulaire, Architecture & React ARN, F-67084 Strasbourg, France |
推荐引用方式 GB/T 7714 | Chen, Xin,Ma, Jing-Jing,Tan, Min,et al. Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase[J]. NUCLEIC ACIDS RESEARCH,2011,39(1):235-247. |
APA | Chen, Xin.,Ma, Jing-Jing.,Tan, Min.,Yao, Peng.,Hu, Qing-Hua.,...&Wang, En-Duo.(2011).Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase.NUCLEIC ACIDS RESEARCH,39(1),235-247. |
MLA | Chen, Xin,et al."Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase".NUCLEIC ACIDS RESEARCH 39.1(2011):235-247. |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 |
修改评论
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。