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A multi-dataset data-collection strategy produces better diffraction data | |
2011 | |
发表期刊 | ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES |
ISSN | 2053-2733 |
卷号 | 67页码:544-549 |
发表状态 | 已发表 |
DOI | 10.1107/S0108767311037469 |
摘要 | A multi-dataset (MDS) data-collection strategy is proposed and analyzed for macromolecular crystal diffraction data acquisition. The theoretical analysis indicated that the MDS strategy can reduce the standard deviation (background noise) of diffraction data compared with the commonly used single-dataset strategy for a fixed X-ray dose. In order to validate the hypothesis experimentally, a data-quality evaluation process, termed a readiness test of the X-ray data-collection system, was developed. The anomalous signals of sulfur atoms in zinc-free insulin crystals were used as the probe to differentiate the quality of data collected using different data-collection strategies. The data-collection results using home-laboratory-based rotating-anode X-ray and synchrotron X-ray systems indicate that the diffraction data collected with the MDS strategy contain more accurate anomalous signals from sulfur atoms than the data collected with a regular data-collection strategy. In addition, the MDS strategy offered more advantages with respect to radiation-damage-sensitive crystals and better usage of rotating-anode as well as synchrotron X-rays. |
学科领域 | Crystallography |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000296789800007 |
出版者 | WILEY-BLACKWELL |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1091 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Liu, Zhi-Jie |
作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA |
推荐引用方式 GB/T 7714 | Liu, Zhi-Jie,Chen, Lirong,Wu, Dong,et al. A multi-dataset data-collection strategy produces better diffraction data[J]. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES,2011,67:544-549. |
APA | Liu, Zhi-Jie.,Chen, Lirong.,Wu, Dong.,Ding, Wei.,Zhang, Hua.,...&Wang, Bi-Cheng.(2011).A multi-dataset data-collection strategy produces better diffraction data.ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES,67,544-549. |
MLA | Liu, Zhi-Jie,et al."A multi-dataset data-collection strategy produces better diffraction data".ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES 67(2011):544-549. |
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