Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function

doi:10.3389/fnmol.2017.00379

	Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function
	Zorzi, Veronica 1,2; Paciello, Fabiola 1; Ziraldo, Gaia 1,2; Peres, Chiara 1; Mazzarda, Flavia 1,3; Nardin, Chiara 1,3; Pasquini, Miriam 1,4; Chiani, Francesco 1; Raspa, Marcello 1; Scavizzi, Ferdinando 1; Carrer, Andrea 5; Crispino, Giulia 5; Ciubotaru, Catalin D.6; Monyer, Hannah 7; Fetoni, Anna R.2; Salvatore, Anna M.1; Mammano, Fabio1,5,8
	2017-11-28
发表期刊	FRONTIERS IN MOLECULAR NEUROSCIENCE
ISSN	1662-5099
卷号	10
发表状态	已发表
DOI	10.3389/fnmol.2017.00379
摘要	Panx1 forms plasma membrane channels in brain and several other organs, including the inner ear. Biophysical properties, activation mechanisms and modulators of Panx1 channels have been characterized in detail, however the impact of Panx1 on auditory function is unclear due to conflicts in published results. To address this issue, hearing performance and cochlear function of the Panx1-/- mouse strain, the first with a reported global ablation of Panx1, were scrutinized. Male and female homozygous (Panx1-/-), hemizygous (Panx1+/-) and their wild type (WT) siblings (Panx1+/+) were used for this study. Successful ablation of Panx1 was confirmed by RT-PCR and Western immunoblotting in the cochlea and brain of Panx1-/- mice. Furthermore, a previously validated Panx1-selective antibody revealed strong immunoreactivity in WT but not in Panx1-/- cochleae. Hearing sensitivity, outer hair cell-based "cochlear amplifier" and cochlear nerve function, analyzed by auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) recordings, were normal in Panx1+/- and Panx1-/- mice. In addition, we determined that global deletion of Panx1 impacts neither on connexin expression, nor on gap-junction coupling in the developing organ of Corti. Finally, spontaneous intercellular Ca2+ signal (ICS) activity in organotypic cochlear cultures, which is key to postnatal development of the organ of Corti and essential for hearing acquisition, was not affected by Panx1 ablation. Therefore, our results provide strong evidence that, in mice, Panx1 is dispensable for hearing acquisition and auditory function.
关键词	cochlea pannexins connexins hair cells non-sensory cells auditory brainstem responses distortion product otoacoustic emissions
收录类别	SCI
语种	英语
资助项目	Fondazione Telethon[GGP13114]
WOS研究方向	Neurosciences & Neurology
WOS类目	Neurosciences
WOS记录号	WOS:000416343500001
出版者	FRONTIERS MEDIA SA
WOS关键词	GAP-JUNCTION PROTEINS ; BRAIN-STEM RESPONSES ; INNER-EAR ; ATP RELEASE ; PANNEXIN CHANNELS ; GENE-EXPRESSION ; HAIR-CELLS ; DEVELOPMENTAL EXPRESSION ; OTOACOUSTIC EMISSIONS ; NONSENSORY CELLS
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/10040
专题	免疫化学研究所_特聘教授组_功能筛选实验室
通讯作者	Mammano, Fabio
作者单位	1.CNR, Inst Cell Biol & Neurobiol, Monterotondo, Italy 2.Catholic Univ, Inst Otolaryngol, Sch Med, Rome, Italy 3.Roma Tre Univ, Dept Sci, Rome, Italy 4.Sapienza Univ Rome, Dept Biol & Biotechnol Charles Darwin, Rome, Italy 5.Univ Padua, Dept Phys & Astron G Galilei, Padua, Italy 6.CNR, Inst Neurosci, Padua Sect, Padua, Italy 7.Heidelberg Univ, Dept Clin Neurobiol, Deutches Krebforschungzentrum, Heidelberg, Germany 8.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China
通讯作者单位	免疫化学研究所
推荐引用方式 GB/T 7714	Zorzi, Veronica,Paciello, Fabiola,Ziraldo, Gaia,et al. Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function[J]. FRONTIERS IN MOLECULAR NEUROSCIENCE,2017,10.
APA	Zorzi, Veronica.,Paciello, Fabiola.,Ziraldo, Gaia.,Peres, Chiara.,Mazzarda, Flavia.,...&Mammano, Fabio.(2017).Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function.FRONTIERS IN MOLECULAR NEUROSCIENCE,10.
MLA	Zorzi, Veronica,et al."Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function".FRONTIERS IN MOLECULAR NEUROSCIENCE 10(2017).