UbcD1 regulates Hedgehog signaling by directly modulating Ci ubiquitination and processing

doi:10.15252/embr.201643289

	UbcD1 regulates Hedgehog signaling by directly modulating Ci ubiquitination and processing
	Pan, Chenyu 1; Xiong, Yue 1; Lv, Xiangdong 1; Xia, Yuanxin 1; Zhang, Shuo 1; Chen, Hao 1; Fan, Jialin 1; Wu, Wenqing 1; Liu, Feng 2; Wu, Hailong 1; Zhou, Zhaocai1,3 ; Zhang, Lei1,3 ; Zhao, Yun1,3
	2017-11
发表期刊	EMBO REPORTS (IF:6.5[JCR-2023],8.3[5-Year])
ISSN	1469-221X
卷号	18 期号:11 页码:1922-1934
发表状态	已发表
DOI	10.15252/embr.201643289
摘要	The Hh pathway controls many morphogenetic processes in metazoans and plays important roles in numerous pathologies and in cancer. Hh signaling is mediated by the activity of the Gli/Ci family of transcription factors. Several studies in Drosophila have shown that ubiquitination by the ubiquitin E3 ligases Slimb and Rdx(Hib) plays a crucial role in controlling Ci stability dependent on the levels of Hh signals. If Hh levels are low, Slimb adds K11- and K48-linked poly-ubiquitin chains on Ci resulting in partial degradation. Ubiquitin E2 enzymes are pivotal in determining the topologies of ubiquitin chains. However, which E2 enzymes participate in the selective ubiquitination-degradation of Ci remains elusive. Here, we find that the E2 enzyme UbcD1 negatively regulates Hh signaling activity in Drosophila wing disks. Genetic and biochemical analyses in wing disks and in cultured cells reveal that UbcD1 directly controls Ci stability. Interestingly, UbcD1 is found to be selectively involved in Slimb-mediated Ci degradation. Finally, we show that the homologs of UbcD1 play a conserved role in modulating Hh signaling in vertebrates.
关键词	Ci Drosophila Hedgehog UbcD1 ubiquitination
收录类别	SCI
语种	英语
资助项目	International Cooperation Program of Bureau of International Cooperation of Chinese Academy of Sciences[153D31KYSB20160137]
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000414346700008
出版者	WILEY
WOS关键词	PROTEIN-KINASE-A ; TARGETS CUBITUS-INTERRUPTUS ; REQUIRES PHOSPHORYLATION ; TRANSCRIPTION FACTOR ; ZEBRAFISH EMBRYO ; REPRESSOR FORMS ; FLOOR PLATE ; BETA-TRCP ; DROSOPHILA ; DEGRADATION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/10036
专题	生命科学与技术学院_特聘教授组_张雷组生命科学与技术学院_特聘教授组_赵允组生命科学与技术学院_特聘教授组_周兆才组
通讯作者	Zhao, Yun
作者单位	1.Univ Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,State Key Lab Cell Biol, CAS Ctr Excellence Mol Cell Sci,Innovat Ctr Cell, Shanghai, Peoples R China 2.Chinese Acad Sci, Inst Zool, State Key Lab Membrane Biol, Beijing, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Pan, Chenyu,Xiong, Yue,Lv, Xiangdong,et al. UbcD1 regulates Hedgehog signaling by directly modulating Ci ubiquitination and processing[J]. EMBO REPORTS,2017,18(11):1922-1934.
APA	Pan, Chenyu.,Xiong, Yue.,Lv, Xiangdong.,Xia, Yuanxin.,Zhang, Shuo.,...&Zhao, Yun.(2017).UbcD1 regulates Hedgehog signaling by directly modulating Ci ubiquitination and processing.EMBO REPORTS,18(11),1922-1934.
MLA	Pan, Chenyu,et al."UbcD1 regulates Hedgehog signaling by directly modulating Ci ubiquitination and processing".EMBO REPORTS 18.11(2017):1922-1934.