Discovery and characterization of potent pan-variant SARS-CoV-2 neutralizing antibodies from individuals with Omicron breakthrough infection

doi:10.1038/s41467-023-39267-x

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	Discovery and characterization of potent pan-variant SARS-CoV-2 neutralizing antibodies from individuals with Omicron breakthrough infection
	Guo, Yu 1,2,3,4,5; Zhang, Guangshun 1,2,4,6,7; Yang, Qi 4; Xie, Xiaowei 3; Lu, Yang 3; Cheng, Xuelian 3; Wang, Hui 5,6; Liang, Jingxi 1,2,8; Tang, Jielin 4; Gao, Yuxin 1,2,6,7; Shang, Hang 1,2,6,7; Dai, Jun 9; Shi, Yongxia 9; Zhou, Jiaxi 3; Zhou, Jun 1,2,3; Guo, Hangtian 8; Yang, Haitao8 ; Qi, Jianwei 3; Liu, Lijun 3; Ma, Shihui 3; Zhang, Biao 3; Huo, Qianyu 3; Xie, Yi 10; Wu, Junping 10; Dong, Fang 1,2,7; Zhang, Song 1,2,7; Lou, Zhiyong 4; Gao, Yan8 ; Song, Zidan 1,2,4,6,7; Wang, Wenming 1,2,6,7; Sun, Zixian 4; Yang, Xiaoming 5,6; Xiong, Dongsheng 3; Liu, Fengjiang 4; Chen, Xinwen 4; Zhu, Ping 3; Wang, Ximo 10; Cheng, Tao 3; Rao, Zihe1,2,4,6,8
	2023-06-15
发表期刊	NATURE COMMUNICATIONS
EISSN	2041-1723
卷号	14 期号:1
发表状态	已发表
DOI	10.1038/s41467-023-39267-x
摘要	The SARS-CoV-2 Omicron variant evades most currently approved neutralizing antibodies (nAbs) and caused drastic decrease of plasma neutralizing activity elicited by vaccination or prior infection, urging the need for the development of pan-variant antivirals. Breakthrough infection induces a hybrid immunological response with potentially broad, potent and durable protection against variants, therefore, convalescent plasma from breakthrough infection may provide a broadened repertoire for identifying elite nAbs. We performed single-cell RNA sequencing (scRNA-seq) and BCR sequencing (scBCR-seq) of B cells from BA.1 breakthrough-infected patients who received 2 or 3 previous doses of inactivated vaccine. Elite nAbs, mainly derived from the IGHV2-5 and IGHV3-66/53 germlines, showed potent neutralizing activity across Wuhan-Hu-1, Delta, Omicron sublineages BA.1 and BA.2 at picomolar NT50 values. Cryo-EM analysis revealed diverse modes of spike recognition and guides the design of cocktail therapy. A single injection of paired antibodies cocktail provided potent protection in the K18-hACE2 transgenic female mouse model of SARS-CoV-2 infection.
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收录类别	SCI
语种	英语
资助项目	National Program on Key Research Project of China["2018YFE0200400","2021YFA1100900","2018YFA0507200"] ; National Natural Science Foundation of China (NSFC)["32271256","81890990","81730006"] ; Key Program of Natural Science Foundation of Tianjin[20JCYBJC01340] ; Haihe Laboratory of Cell Ecosystem Innovation Fund[22HHXBSS00001] ; Science and Technology Project of Tianjin[22ZYJDSS00080] ; Chinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Sciences[2021-I2M-1-040] ; Non-CAMS Fundamental Research Funds for Central Research Institutes[3332021093] ; Basic and Applied Basic Research Projects of Guangzhou Basic Research Program[2023A04J0161] ; Emergency Key Program of Guangzhou Laboratory[EKPGL2021008] ; R&D Program of Guangzhou Laboratory["SRPG22-002","SRPG22-003"]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001026275700020
出版者	NATURE PORTFOLIO
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/325777
专题	免疫化学研究所免疫化学研究所_特聘教授组_饶子和组免疫化学研究所_PI研究组_杨海涛组
通讯作者	Guo, Yu; Yang, Qi; Yang, Xiaoming; Xiong, Dongsheng; Liu, Fengjiang; Chen, Xinwen; Zhu, Ping; Wang, Ximo; Cheng, Tao; Rao, Zihe
作者单位	1.Nankai Univ, State Key Lab Med Chem Biol, 38 Tongyan Rd, Tianjin 300071, Peoples R China 2.Nankai Univ, Coll Life Sci, 38 Tongyan Rd, Tianjin 300071, Peoples R China 3.Chinese Acad Med Sci & Peking Union Med Coll, Inst Hematol & Blood Dis Hosp, Haihe Lab Cell Ecosyst, State Key Lab Expt Hematol,Natl Clin Res Ctr Bloo, Tianjin 300020, Peoples R China 4.Guangzhou Lab, Guangzhou, Guangdong, Peoples R China 5.Beijing Inst Biol Prod Co Ltd, China Natl Biotech Grp, Beijing 100176, Peoples R China 6.Nankai Univ, CNBG Nankai Joint Res Ctr, 94Weijin Rd, Tianjin 300071, Peoples R China 7.Nankai Univ, Frontiers Sci Ctr Cell Responses, 94 Weijin Rd, Tianjin 300071, Peoples R China 8.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China 9.Guangzhou Customs Dist Technol Ctr, Guangzhou 510700, Peoples R China 10.Tianjin Haihe Hosp, Jingu Rd, Tianjin 300071, Peoples R China
通讯作者单位	免疫化学研究所
推荐引用方式 GB/T 7714	Guo, Yu,Zhang, Guangshun,Yang, Qi,et al. Discovery and characterization of potent pan-variant SARS-CoV-2 neutralizing antibodies from individuals with Omicron breakthrough infection[J]. NATURE COMMUNICATIONS,2023,14(1).
APA	Guo, Yu.,Zhang, Guangshun.,Yang, Qi.,Xie, Xiaowei.,Lu, Yang.,...&Rao, Zihe.(2023).Discovery and characterization of potent pan-variant SARS-CoV-2 neutralizing antibodies from individuals with Omicron breakthrough infection.NATURE COMMUNICATIONS,14(1).
MLA	Guo, Yu,et al."Discovery and characterization of potent pan-variant SARS-CoV-2 neutralizing antibodies from individuals with Omicron breakthrough infection".NATURE COMMUNICATIONS 14.1(2023).