Structures of fungal and plant acetohydroxyacid synthases

doi:10.1038/s41586-020-2514-3

	Structures of fungal and plant acetohydroxyacid synthases
	Lonhienne, Thierry 1; Low, Yu Shang 1; Garcia, Mario D.1; Croll, Tristan 2; Gao, Yan3 ; Wang, Quan3 ; Brillault, Lou 4; Williams, Craig M.1; Fraser, James A.1; McGeary, Ross P.1; West, Nicholas P.1; Landsberg, Michael J.1; Rao, Zihe3,5,6 ; Schenk, Gerhard 1; Guddat, Luke W.1
	2020-10-08
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
卷号	586 期号:7828 页码:317-+
发表状态	已发表
DOI	10.1038/s41586-020-2514-3
摘要	Acetohydroxyacid synthase (AHAS), also known as acetolactate synthase, is a flavin adenine dinucleotide-, thiamine diphosphate- and magnesium-dependent enzyme that catalyses the first step in the biosynthesis of branched-chain amino acids(1). It is the target for more than 50 commercial herbicides(2). AHAS requires both catalytic and regulatory subunits for maximal activity and functionality. Here we describe structures of the hexadecameric AHAS complexes ofSaccharomyces cerevisiaeand dodecameric AHAS complexes ofArabidopsis thaliana. We found that the regulatory subunits of these AHAS complexes form a core to which the catalytic subunit dimers are attached, adopting the shape of a Maltese cross. The structures show how the catalytic and regulatory subunits communicate with each other to provide a pathway for activation and for feedback inhibition by branched-chain amino acids. We also show that the AHAS complex ofMycobacterium tuberculosisadopts a similar structure, thus demonstrating that the overall AHAS architecture is conserved across kingdoms. Structures of the acetohydroxyacid synthase complexes ofSaccharomyces cerevisiaeandArabidopsis thalianaprovide insights into the biosynthesis of and feedback inhibition by branched-chain amino acids.
URL	查看原文
收录类别	SCIE ; SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000567779800001
出版者	NATURE RESEARCH
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/243270
专题	免疫化学研究所_特聘教授组_饶子和组免疫化学研究所_PI研究组_王权组
通讯作者	Lonhienne, Thierry; Guddat, Luke W.
作者单位	1.Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld, Australia; 2.Univ Cambridge, Cambridge Inst Med Res, Cambridge, England; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China; 4.Univ Queensland, Ctr Microscopy & Microanal, Brisbane, Qld, Australia; 5.Nankai Univ, Coll Life Sci, State Key Lab Med Chem Biol, Tianjin, Peoples R China; 6.Tsinghua Univ, Lab Struct Biol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Lonhienne, Thierry,Low, Yu Shang,Garcia, Mario D.,et al. Structures of fungal and plant acetohydroxyacid synthases[J]. NATURE,2020,586(7828):317-+.
APA	Lonhienne, Thierry.,Low, Yu Shang.,Garcia, Mario D..,Croll, Tristan.,Gao, Yan.,...&Guddat, Luke W..(2020).Structures of fungal and plant acetohydroxyacid synthases.NATURE,586(7828),317-+.
MLA	Lonhienne, Thierry,et al."Structures of fungal and plant acetohydroxyacid synthases".NATURE 586.7828(2020):317-+.