The m(6)A reader YTHDF1 promotes osteogenesis of bone marrow mesenchymal stem cells through translational control of ZNF839

doi:10.1038/s41419-021-04312-4

	The m(6)A reader YTHDF1 promotes osteogenesis of bone marrow mesenchymal stem cells through translational control of ZNF839
	Liu, Tao 1,2; Zheng, Xinfeng 1; Wang, Chenglong 3; Wang, Chuandong 3; Jiang, Shengdan 1; Li, Bo 1; Chen, Pengbo 1; Xu, Wenning 1; Zheng, Huoliang 1; Yang, Runze 1; Huang, Xingxu4 ; Zhang, Xiaoling 3; Jiang, Leisheng 1
	2021-11-12
发表期刊	CELL DEATH & DISEASE
ISSN	2041-4889
卷号	12 期号:11
DOI	10.1038/s41419-021-04312-4
摘要	N6-methyladenosine (m(6)A) is required for differentiation of human bone marrow mesenchymal stem cells (hBMSCs). However, its intrinsic mechanisms are largely unknown. To identify the possible role of m(6)A binding protein YTHDF1 in hBMSCs osteogenesis in vivo, we constructed Ythdf1 KO mice and showed that depletion of Ythdf1 would result in decreased bone mass in vivo. Both deletion of Ythdf1 in mouse BMSCs and shRNA-mediated knockdown of YTHDF1 in hBMSCs prevented osteogenic differentiation of cells in vitro. Using methylated RNA immunoprecipitation (Me-RIP) sequencing and RIP-sequencing, we found that ZNF839 (a zinc finger protein) served as a target of YTHDF1. We also verified its mouse homolog, Zfp839, was translationally regulated by Ythdf1 in an m(6)A-dependent manner. Zfp839 potentiated BMSC osteogenesis by interacting with and further enhancing the transcription activity of Runx2. These findings should improve our understanding of the mechanism of BMSC osteogenesis regulation and provide new ideas for the prevention and treatment of osteoporosis.
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收录类别	SCIE
语种	英语
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000717996100002
出版者	SPRINGERNATURE
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/131968
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Zhang, Xiaoling; Jiang, Leisheng
作者单位	1.Shanghai Jiao Tong Univ, Spine Ctr, Xinhua Hosp, Sch Med, Shanghai 200092, Peoples R China; 2.Jiangsu Univ, Dept Orthoped Surg, Affiliated Peoples Hosp, Zhenjiang 212002, Jiangsu, Peoples R China; 3.Shanghai Jiao Tong Univ, Dept Orthoped Surg, Xinhua Hosp, Sch Med, Shanghai 200092, Peoples R China; 4.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Liu, Tao,Zheng, Xinfeng,Wang, Chenglong,et al. The m(6)A reader YTHDF1 promotes osteogenesis of bone marrow mesenchymal stem cells through translational control of ZNF839[J]. CELL DEATH & DISEASE,2021,12(11).
APA	Liu, Tao.,Zheng, Xinfeng.,Wang, Chenglong.,Wang, Chuandong.,Jiang, Shengdan.,...&Jiang, Leisheng.(2021).The m(6)A reader YTHDF1 promotes osteogenesis of bone marrow mesenchymal stem cells through translational control of ZNF839.CELL DEATH & DISEASE,12(11).
MLA	Liu, Tao,et al."The m(6)A reader YTHDF1 promotes osteogenesis of bone marrow mesenchymal stem cells through translational control of ZNF839".CELL DEATH & DISEASE 12.11(2021).