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The amyloid structure of mouse RIPK3 (receptor interacting protein kinase 3) in cell necroptosis | |
2021-03-12 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
卷号 | 12期号:1页码:#VALUE! |
发表状态 | 已发表 |
DOI | 10.1038/s41467-021-21881-2 |
摘要 | RIPK3 amyloid complex plays crucial roles during TNF-induced necroptosis and in response to immune defense in both human and mouse. Here, we have structurally characterized mouse RIPK3 homogeneous self-assembly using solid-state NMR, revealing a well-ordered N-shaped amyloid core structure featured with 3 parallel in-register beta -sheets. This structure differs from previously published human RIPK1/RIPK3 hetero-amyloid complex structure, which adopted a serpentine fold. Functional studies indicate both RIPK1-RIPK3 binding and RIPK3 amyloid formation are essential but not sufficient for TNF-induced necroptosis. The structural integrity of RIPK3 fibril with three beta -strands is necessary for signaling. Molecular dynamics simulations with a mouse RIPK1/RIPK3 model indicate that the hetero-amyloid is less stable when adopting the RIPK3 fibril conformation, suggesting a structural transformation of RIPK3 from RIPK1-RIPK3 binding to RIPK3 amyloid formation. This structural transformation would provide the missing link connecting RIPK1-RIPK3 binding to RIPK3 homo-oligomer formation in the signal transduction. Receptor Interacting Protein Kinase 3 (RIPK3) has a key role in TNF-induced necroptosis. Here, the authors combine solid state NMR measurements, MD simulations and cell based assays to characterize mouse RIPK3 and they present the structure of the RIPK3 amyloid core. |
URL | 查看原文 |
收录类别 | SCI ; SCIE |
语种 | 英语 |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000630419400018 |
出版者 | NATURE RESEARCH |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/125742 |
专题 | 生命科学与技术学院_博士生 生命科学与技术学院_PI研究组_王华翌组 生命科学与技术学院_PI研究组_陆珺霞组 生命科学与技术学院_公共科研平台_分子细胞学平台 生命科学与技术学院_硕士生 |
共同第一作者 | Hu, Hong |
通讯作者 | Wang, Hua-Yi; Lu, Jun-Xia |
作者单位 | 1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 2.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China; 4.NIH, Lab Imaging Sci, Off Intramural Res, Ctr Informat Technol, Bldg 10, Bethesda, MD 20892 USA |
第一作者单位 | 生命科学与技术学院 |
通讯作者单位 | 生命科学与技术学院 |
第一作者的第一单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Wu, Xia-Lian,Hu, Hong,Dong, Xing-Qi,et al. The amyloid structure of mouse RIPK3 (receptor interacting protein kinase 3) in cell necroptosis[J]. NATURE COMMUNICATIONS,2021,12(1):#VALUE!. |
APA | Wu, Xia-Lian.,Hu, Hong.,Dong, Xing-Qi.,Zhang, Jing.,Wang, Jian.,...&Lu, Jun-Xia.(2021).The amyloid structure of mouse RIPK3 (receptor interacting protein kinase 3) in cell necroptosis.NATURE COMMUNICATIONS,12(1),#VALUE!. |
MLA | Wu, Xia-Lian,et al."The amyloid structure of mouse RIPK3 (receptor interacting protein kinase 3) in cell necroptosis".NATURE COMMUNICATIONS 12.1(2021):#VALUE!. |
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