Late endosomes promote microglia migration via cytosolic translocation of immature protease cathD

doi:10.1126/sciadv.aba5783

	Late endosomes promote microglia migration via cytosolic translocation of immature protease cathD
	Liu, Yi-Jun 1,2; Zhang, Ting 1,2; Cheng, Daxiao 1,2; Yang, Junhua 1,2; Chen, Sicong 3; Wang, Xingyue 1,2; Li, Xia 1,2; Duan, Duo 1,2; Lou, Huifang 1,2; Zhu, Liya 1,2; Luo, Jianhong 1,2; Ho, Margaret S.4 ; Wang, Xiao-Dong 1,2,5; Duan, Shumin 1,2,6
	2020-12
发表期刊	SCIENCE ADVANCES
ISSN	2375-2548
卷号	6 期号:50
发表状态	已发表
DOI	10.1126/sciadv.aba5783
摘要	Organelle transport requires dynamic cytoskeleton remodeling, but whether cytoskeletal dynamics are, in turn, regulated by organelles remains elusive. Here, we demonstrate that late endosomes, a type of prelysosomal organelles, facilitate actin-cytoskeleton remodeling via cytosolic translocation of immature protease cathepsin D (cathD) during microglia migration. After cytosolic translocation, late endosome-derived cathD juxtaposes actin filaments at the leading edge of lamellipodia. Suppressing cathD expression or blocking its cytosolic translocation impairs the maintenance but not the initiation of lamellipodial extension. Moreover, immature cathD balances the activity of the actin-severing protein cofilin to maintain globular-actin (G-actin) monomer pool for local actin recycling. Our study identifies cathD as a key lysosomal molecule that unconventionally contributes to actin cytoskeleton remodeling via cytosolic translocation during adenosine triphosphate-evoked microglia migration.
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收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	National Key Research and Development Program of China[2016YFA0501000] ; National Natural Science Foundation of China[81821091][31501128][31490592][31871039][81761138044][81527901][81801330][91832000] ; Key Research and Development Program of Zhejiang Province[2020C03009] ; Science and Technology Planning Project of Guangdong Province[2018B030331001] ; Key Realm R&D Program of Guangdong Province[2019B030335001] ; Chinese Ministry of Education Project 111 Program[B13026]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000597410300002
出版者	AMER ASSOC ADVANCEMENT SCIENCE
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/124910
专题	生命科学与技术学院_PI研究组_何淑君组
通讯作者	Liu, Yi-Jun; Ho, Margaret S.; Wang, Xiao-Dong; Duan, Shumin
作者单位	1.Zhejiang Univ, NHC & CAMS Key Lab Med Neurobiol, Sch Brain Sci & Brian Med, Dept Neurobiol,Sch Med, Hangzhou 310058, Peoples R China 2.Zhejiang Univ, MOE, Frontier Sci Ctr Brain Res & Brain Machine Integr, Sch Med, Hangzhou 310058, Peoples R China 3.Zhejiang Univ, Affiliated Hosp 2, Clin Res Ctr, Sch Med, Hangzhou 310009, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 5.Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Psychiat, Sch Med, Hangzhou 310016, Peoples R China 6.Zhejiang Univ, Mental Hlth Ctr, Sch Med, Hangzhou, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Liu, Yi-Jun,Zhang, Ting,Cheng, Daxiao,et al. Late endosomes promote microglia migration via cytosolic translocation of immature protease cathD[J]. SCIENCE ADVANCES,2020,6(50).
APA	Liu, Yi-Jun.,Zhang, Ting.,Cheng, Daxiao.,Yang, Junhua.,Chen, Sicong.,...&Duan, Shumin.(2020).Late endosomes promote microglia migration via cytosolic translocation of immature protease cathD.SCIENCE ADVANCES,6(50).
MLA	Liu, Yi-Jun,et al."Late endosomes promote microglia migration via cytosolic translocation of immature protease cathD".SCIENCE ADVANCES 6.50(2020).