Synphilin-1 Interacts with AMPK and Increases AMPK Phosphorylation

doi:10.3390/ijms21124352

	Synphilin-1 Interacts with AMPK and Increases AMPK Phosphorylation
	Li, Tianxia 1,2; Liu, Jingnan2,4 ; Guo, Gongbo 1; Ning, Bo 1,3; Li, Xueping 1,3; Zhu, Guangjing 1; Yang, Dejun 1; Moran, Timothy H.1; Smith, Wanli W.1
	2020-06
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
卷号	21 期号:12
发表状态	已发表
DOI	10.3390/ijms21124352
摘要	A role for the cytoplasmic protein synphilin-1 in regulating energy balance has been demonstrated recently. Expression of synphilin-1 increases ATP levels in cultured cells. However, the mechanism by which synphilin-1 alters cellular energy status is unknown. Here, we used cell models and biochemical approaches to investigate the cellular functions of synphilin-1 on the AMP-activated protein kinase (AMPK) signaling pathway, which may affect energy balance. Overexpression of synphilin-1 increased AMPK phosphorylation (activation). Moreover, synphilin-1 interacted with AMPK by co-immunoprecipitation and GST (glutathione S-transferase) pull-down assays. Knockdown of synphilin-1 reduced AMPK phosphorylation. Overexpression of synphilin-1 also altered AMPK downstream signaling, i.e., a decrease in acetyl CoA carboxylase (ACC) phosphorylation, and an increase in p70S6K phosphorylation. Treatment of compound C (an AMPK inhibitor) reduced synphilin-1 binding with AMPK. In addition, compound C diminished synphilin-1-induced AMPK phosphorylation, and the increase in cellular ATP (adenosine triphosphate) levels. Our results demonstrated that synphilin-1 couples with AMPK, and they exert mutual effects on each other to regulate cellular energy status. These findings not only identify novel cellular actions of synphilin-1, but also provide new insights into the roles of synphilin-1 in regulating energy currency, ATP.
关键词	synphilin-1 AMPK ATP energy balance protein interaction
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Institutes of Health[DK083410]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000549501400001
出版者	MDPI
WOS关键词	ACTIVATED PROTEIN-KINASE ; ALPHA-SYNUCLEIN ; INCLUSION FORMATION ; PARKINSONS-DISEASE ; LEWY BODIES ; CELL-GROWTH ; FOOD-INTAKE ; NUTRIENT ; SIGNALS ; OBESITY
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122485
专题	生命科学与技术学院_PI研究组_刘冀珑组
通讯作者	Smith, Wanli W.
作者单位	1.Johns Hopkins Univ, Sch Med, Dept Psychiat, Baltimore, MD 21205 USA 2.Univ Maryland, Sch Pharm, Dept Pharmaceut Sci, Baltimore, MD 21201 USA 3.Xian Med Coll, Inst Obes & Metab Dis, Sch Clin Med, Xian 71000, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Li, Tianxia,Liu, Jingnan,Guo, Gongbo,et al. Synphilin-1 Interacts with AMPK and Increases AMPK Phosphorylation[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(12).
APA	Li, Tianxia.,Liu, Jingnan.,Guo, Gongbo.,Ning, Bo.,Li, Xueping.,...&Smith, Wanli W..(2020).Synphilin-1 Interacts with AMPK and Increases AMPK Phosphorylation.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(12).
MLA	Li, Tianxia,et al."Synphilin-1 Interacts with AMPK and Increases AMPK Phosphorylation".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.12(2020).