In vitro expression and analysis of the 826 human G protein-coupled receptors

doi:10.1007/s13238-016-0263-8

KMS > iHuman研究所 > 特聘教授组 > Kurt Wuthrich组

	In vitro expression and analysis of the 826 human G protein-coupled receptors
	Lv, Xuechen1 ; Liu, Junlin1 ; Shi, Qiaoyun1 ; Tan, Qiwen1 ; Wu, Dong1 ; Skinner, John J.1 ; Walker, Angela L.2; Zhao, Lixia1 ; Gu, Xiangxiang1 ; Chen, Na1 ; Xue, Lu1 ; Si, Pei1 ; Zhang, Lu1 ; Wang, Zeshi1 ; Katritch, Vsevolod 2; Liu, Zhi-Jie1 ; Stevens, Raymond C.1
	2016-05
发表期刊	PROTEIN & CELL
ISSN	1674-800X
卷号	7 期号:5 页码:325-337
发表状态	已发表
DOI	10.1007/s13238-016-0263-8
摘要	G protein-coupled receptors (GPCRs) are involved in all humanphysiological systems where they are responsible for transducing extracellular signals into cells. GPCRs signal in response to a diverse array of stimuli including light, hormones, and lipids, where these signals affect downstream cascades to impact both health and disease states. Yet, despite their importance as therapeutic targets, detailed molecular structures of only 30 GPCRs have been determined to date. A key challenge to their structure determination is adequate protein expression. Here we report the quantification of protein expression in an insect cell expression system for all 826 human GPCRs using two different fusion constructs. Expression characteristics are analyzed in aggregate and among each of the five distinct subfamilies. These data can be used to identify trends related to GPCR expression between different fusion constructs and between different GPCR families, and to prioritize lead candidates for future structure determination feasibility.
关键词	G protein-coupled receptors insect protein expression surface expression analysis fusion construct
收录类别	SCI
语种	英语
资助项目	National Basic Research Program (973 Program)[2014CB910400] ; National Basic Research Program (973 Program)[2015CB910104]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000379180100003
出版者	HIGHER EDUCATION PRESS
WOS关键词	CRYSTAL-STRUCTURE ; GPCR ; GLYCOSYLATION
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1061
专题	iHuman研究所_特聘教授组_Kurt Wuthrich组 iHuman研究所 iHuman研究所_特聘教授组_Raymond Stevens组免疫化学研究所_公共科研平台_蛋白表达与制备平台 iHuman研究所_公共科研平台_Assay及筛选平台 iHuman研究所_公共科研平台_动物细胞培养和蛋白纯化平台 iHuman研究所_公共科研平台_昆虫细胞培养平台 iHuman研究所_公共科研平台_克隆平台 iHuman研究所_PI研究组_刘志杰组 iHuman研究所_PI研究组_徐菲组 iHuman研究所_PI研究组_陶厚朝组生命科学与技术学院_硕士生
通讯作者	Stevens, Raymond C.
作者单位	1.ShanghaiTech Univ, IHuman Inst, Shanghai 201210, Peoples R China 2.Univ Southern Calif, Dept Biol Sci, Bridge Inst, Los Angeles, CA 90089 USA
第一作者单位	iHuman研究所
通讯作者单位	iHuman研究所
第一作者的第一单位	iHuman研究所
推荐引用方式 GB/T 7714	Lv, Xuechen,Liu, Junlin,Shi, Qiaoyun,et al. In vitro expression and analysis of the 826 human G protein-coupled receptors[J]. PROTEIN & CELL,2016,7(5):325-337.
APA	Lv, Xuechen.,Liu, Junlin.,Shi, Qiaoyun.,Tan, Qiwen.,Wu, Dong.,...&Stevens, Raymond C..(2016).In vitro expression and analysis of the 826 human G protein-coupled receptors.PROTEIN & CELL,7(5),325-337.
MLA	Lv, Xuechen,et al."In vitro expression and analysis of the 826 human G protein-coupled receptors".PROTEIN & CELL 7.5(2016):325-337.