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Establishing a High-Yield
Bacillus subtilis
-Based Cell-Free Protein Synthesis System for In Vitro Prototyping and Natural Product Biosynthesis
期刊论文
ACS SYNTHETIC BIOLOGY, 2025
作者:
Ji, Xiangyang
;
Liu, Wan-Qiu
Adobe PDF(2808Kb)
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收藏
|
浏览/下载:8/1
|
提交时间:2025/04/20
cell-free protein synthesis
T7 RNA polymerase
Bacillus subtilis
genetic part prototyping
natural product biosynthesis
synthetic biology
Chitin-Functionalized Cell-Free System Enables Sustainable Biocatalysis and Gene Expression
期刊论文
ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 2024, 卷号: 12, 期号: 50, 页码: 18219-18230
作者:
Lu, Yicong
;
Zheng, Xiao
;
Liu, Wan-Qiu
;
Ling, Shengjie
;
Li, Jian
Adobe PDF(4458Kb)
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收藏
|
浏览/下载:185/2
|
提交时间:2024/12/17
cell-free protein synthesis
chitin
chitin-bindingdomain
immobilization
biocatalysis
Enzyme-Polymer-Conjugate-Based Pickering Emulsions for Cell-Free Expression and Cascade Biotransformation
期刊论文
ANGEWANDTE CHEMIE INTERNATIONAL EDITION, 2023, 卷号: 62, 期号: 52
作者:
Haofan Lu
;
Jingping Ouyang
;
Wan-Qiu Liu
;
Changzhu Wu
;
Jian Li
Adobe PDF(5016Kb)
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收藏
|
浏览/下载:325/2
|
提交时间:2023/11/28
Biochemistry
Biosynthesis
Catalysis
Cells
Cytology
Emulsification
Ostwald ripening
Aqueous phasis
Biocatalysis
Cascade reactions
Catalytic performance
Cell free protein synthesis
Cell-free expression
Enzyme-polymer conjugate
Non-canonical amino acids
Pickering emulsions
Polymer conjugate
Expanding the toolbox of probiotic
Escherichia coli
Nissle 1917 for synthetic biology
期刊论文
BIOTECHNOLOGY JOURNAL, 2023, 卷号: 19, 期号: 1
作者:
Fang Ba
;
Yufei Zhang
;
Xiangyang Ji
;
Wan-Qiu Liu
;
Shengjie Ling
Adobe PDF(2236Kb)
|
收藏
|
浏览/下载:1607/833
|
提交时间:2023/10/13
cell-free protein synthesis
cryptic plasmid
Escherichia coli
Nissle 1917
integrase
synthetic biology
Expanding the toolbox of probiotic Escherichia coli Nissle 1917 for synthetic biology
预印本
2023
作者:
Ba, Fang
;
Zhang, Yufei
;
Ji, Xiangyang
;
Liu, Wan-Qiu
;
Ling, Shengjie
Adobe PDF(2683Kb)
|
收藏
|
浏览/下载:320/0
|
提交时间:2024/01/09
synthetic biology
probiotic engineering
Escherichia coli Nissle 1917
cryptic plasmid
integrase
cell-free protein synthesis
Cell-free metabolic engineering enables selective biotransformation of fatty acids to value-added chemicals
期刊论文
METABOLIC ENGINEERING COMMUNICATIONS;, 2023, 卷号: 16
作者:
Liu, Yushi
;
Liu, Wan-Qiu
;
Huang, Shuhui
;
Xu, Huiling
;
Lu, Haofan
Adobe PDF(4939Kb)
|
收藏
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浏览/下载:378/0
|
提交时间:2023/03/24
Biocatalysis
Biotransformation
Cell-free metabolic engineering
Cell-free protein synthesis
Fatty acids
Combining Cell-Free Expression and Multifactor Optimization for Enhanced Biosynthesis of Cinnamyl Alcohol
期刊论文
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2023, 卷号: 71, 期号: 22, 页码: 8551-8557
作者:
Zhang, Ren
;
Liu, Wan-Qiu
;
Ling, Shengjie
;
Li, Jian
Adobe PDF(1371Kb)
|
收藏
|
浏览/下载:244/0
|
提交时间:2023/05/29
cell-free protein synthesis
biotransformation
cinnamyl alcohol
multifactoroptimization
Cell-Free Biosynthesis of Lysine-Derived Unnatural Amino Acids with Chloro, Alkene, and Alkyne Groups
期刊论文
ACS SYNTHETIC BIOLOGY, 2023, 卷号: 12, 期号: 4
作者:
Chen, Yilin
;
Liu, Wan-Qiu
;
Zheng, Xiao
;
Liu, Yifan
;
Ling, Shengjie
Adobe PDF(3206Kb)
|
收藏
|
浏览/下载:373/1
|
提交时间:2023/05/08
cell-free protein synthesis
unnatural amino acids
natural products
in vitro biosynthesis
synthetic biology
Cell-Free Expression of a Therapeutic Protein Serratiopeptidase
期刊论文
MOLECULES, 2023, 卷号: 28, 期号: 7, 页码: 3132
作者:
Meng, Yaru
;
Yang, Miaomiao
;
Liu, Wanqiu
;
Li, Jian
Adobe PDF(1924Kb)
|
收藏
|
浏览/下载:492/0
|
提交时间:2023/05/09
cell-free protein synthesis
therapeutic proteins
serratiopeptidase
enzymatic activity
cell-free synthetic biology
Permeability-Engineered Compartmentalization Enables In Vitro Reconstitution of Sustained Synthetic Biology Systems
期刊论文
ADVANCED SCIENCE, 2022
作者:
Li, Luyao
;
Zhang, Rong
;
Chen, Long
;
Tian, Xintong
;
Li, Ting
Adobe PDF(3548Kb)
|
收藏
|
浏览/下载:472/1
|
提交时间:2022/10/18
biomaterials
cell-free protein synthesis
compartmentalization
synthetic biology
whole-cell biosensors
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