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YTHDC1 mediates nuclear export of N-6 - methyladenosine methylated mRNAs | |
2017-10-06 | |
发表期刊 | ELIFE |
ISSN | 2050-084X |
卷号 | 6 |
发表状态 | 已发表 |
DOI | 10.7554/eLife.31311 |
摘要 | N-6-methyladenosine (m(6)A) is the most abundant internal modification of eukaryotic messenger RNA (mRNA) and plays critical roles in RNA biology. The function of this modification is mediated by m(6)A-selective 'reader' proteins of the YTH family, which incorporate m(6)A-modified mRNAs into pathways of RNA metabolism. Here, we show that the m(6)A-binding protein YTHDC1 mediates export of methylated mRNA from the nucleus to the cytoplasm in HeLa cells. Knockdown of YTHDC1 results in an extended residence time for nuclear m(6)A-containing mRNA, with an accumulation of transcripts in the nucleus and accompanying depletion within the cytoplasm. YTHDC1 interacts with the splicing factor and nuclear export adaptor protein SRSF3, and facilitates RNA binding to both SRSF3 and NXF1. This role for YTHDC1 expands the potential utility of chemical modification of mRNA, and supports an emerging paradigm of m(6) A as a distinct biochemical entity for selective processing and metabolism of mammalian mRNAs. |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[31171377] ; National Natural Science Foundation of China[31471400] |
WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
WOS类目 | Biology |
WOS记录号 | WOS:000413185800001 |
出版者 | ELIFE SCIENCES PUBLICATIONS LTD |
WOS关键词 | M(6)A RNA ; GENE-EXPRESSION ; BINDING ; TRANSCRIPTION ; TRANSLATION ; METHYLOMES ; PROTEINS ; LINK ; M6A |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/9925 |
专题 | 生命科学与技术学院_PI研究组_黄行许组 |
通讯作者 | Shen, Bin; He, Chuan |
作者单位 | 1.Univ Chicago, Dept Chem, 5735 S Ellis Ave, Chicago, IL 60637 USA 2.Univ Chicago, Dept Biochem & Mol Biol, 920 E 58Th St, Chicago, IL 60637 USA 3.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA 4.Univ Chicago, Howard Hughes Med Inst, 5841 S Maryland Ave, Chicago, IL 60637 USA 5.Univ Chicago, Med Scientist Traning Program, Chicago, IL 60637 USA 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 7.Nanjing Med Univ, State Key Lab Reprod Med, Dept Histol & Embryol, Nanjing, Jiangsu, Peoples R China |
推荐引用方式 GB/T 7714 | Roundtree, Ian A.,Luo, Guan-Zheng,Zhang, Zijie,et al. YTHDC1 mediates nuclear export of N-6 - methyladenosine methylated mRNAs[J]. ELIFE,2017,6. |
APA | Roundtree, Ian A..,Luo, Guan-Zheng.,Zhang, Zijie.,Wang, Xiao.,Zhou, Tao.,...&He, Chuan.(2017).YTHDC1 mediates nuclear export of N-6 - methyladenosine methylated mRNAs.ELIFE,6. |
MLA | Roundtree, Ian A.,et al."YTHDC1 mediates nuclear export of N-6 - methyladenosine methylated mRNAs".ELIFE 6(2017). |
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