YTHDC1 mediates nuclear export of N-6 - methyladenosine methylated mRNAs

doi:10.7554/eLife.31311

	YTHDC1 mediates nuclear export of N-6 - methyladenosine methylated mRNAs
	Roundtree, Ian A.1,2,3,4,5; Luo, Guan-Zheng 1,2,3,4; Zhang, Zijie 1,2,3,4; Wang, Xiao 1,2,3,4; Zhou, Tao6 ; Cui, Yiquang 7; Sha, Jiahao 7; Huang, Xingxu6 ; Guerrero, Laura 1,2,3,4; Xie, Phil 1,2,3,4; He, Emily 1,2,3,4; Shen, Bin 7; He, Chuan 1,2,3,4
	2017-10-06
Source Publication	ELIFE
ISSN	2050-084X
Volume	6
Status	已发表
DOI	10.7554/eLife.31311
Abstract	N-6-methyladenosine (m(6)A) is the most abundant internal modification of eukaryotic messenger RNA (mRNA) and plays critical roles in RNA biology. The function of this modification is mediated by m(6)A-selective 'reader' proteins of the YTH family, which incorporate m(6)A-modified mRNAs into pathways of RNA metabolism. Here, we show that the m(6)A-binding protein YTHDC1 mediates export of methylated mRNA from the nucleus to the cytoplasm in HeLa cells. Knockdown of YTHDC1 results in an extended residence time for nuclear m(6)A-containing mRNA, with an accumulation of transcripts in the nucleus and accompanying depletion within the cytoplasm. YTHDC1 interacts with the splicing factor and nuclear export adaptor protein SRSF3, and facilitates RNA binding to both SRSF3 and NXF1. This role for YTHDC1 expands the potential utility of chemical modification of mRNA, and supports an emerging paradigm of m(6) A as a distinct biochemical entity for selective processing and metabolism of mammalian mRNAs.
Indexed By	SCI
Language	英语
Funding Project	National Natural Science Foundation of China[31171377] ; National Natural Science Foundation of China[31471400]
WOS Research Area	Life Sciences & Biomedicine - Other Topics
WOS Subject	Biology
WOS ID	WOS:000413185800001
Publisher	ELIFE SCIENCES PUBLICATIONS LTD
WOS Keyword	M(6)A RNA ; GENE-EXPRESSION ; BINDING ; TRANSCRIPTION ; TRANSLATION ; METHYLOMES ; PROTEINS ; LINK ; M6A
Original Document Type	Article
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Document Type	期刊论文
Identifier	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/9925
Collection	生命科学与技术学院_PI研究组_黄行许组
Corresponding Author	Shen, Bin; He, Chuan
Affiliation	1.Univ Chicago, Dept Chem, 5735 S Ellis Ave, Chicago, IL 60637 USA 2.Univ Chicago, Dept Biochem & Mol Biol, 920 E 58Th St, Chicago, IL 60637 USA 3.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA 4.Univ Chicago, Howard Hughes Med Inst, 5841 S Maryland Ave, Chicago, IL 60637 USA 5.Univ Chicago, Med Scientist Traning Program, Chicago, IL 60637 USA 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 7.Nanjing Med Univ, State Key Lab Reprod Med, Dept Histol & Embryol, Nanjing, Jiangsu, Peoples R China
Recommended Citation GB/T 7714	Roundtree, Ian A.,Luo, Guan-Zheng,Zhang, Zijie,et al. YTHDC1 mediates nuclear export of N-6 - methyladenosine methylated mRNAs[J]. ELIFE,2017,6.
APA	Roundtree, Ian A..,Luo, Guan-Zheng.,Zhang, Zijie.,Wang, Xiao.,Zhou, Tao.,...&He, Chuan.(2017).YTHDC1 mediates nuclear export of N-6 - methyladenosine methylated mRNAs.ELIFE,6.
MLA	Roundtree, Ian A.,et al."YTHDC1 mediates nuclear export of N-6 - methyladenosine methylated mRNAs".ELIFE 6(2017).

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