Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation

doi:10.1038/s41598-017-13471-4

	Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation
	Li, Haisen 1,6; Guo, Ao 1; Xie, Zhenfei 1; Tu, Wanzhi4 ; Yu, Jiali 3; Wang, Huihan 3; Zhao, Jian1 ; Zhong, Cuiqing 1; Kang, Jiuhong 5; Li, Jinsong 1; Huang, Shichao 1,2; Shen, Li 3
	2017-10-16
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	7
发表状态	已发表
DOI	10.1038/s41598-017-13471-4
摘要	Mammalian haploid embryonic stem cells (haESCs) provide new possibilities for large-scale genetic screens because they bear only one copy of each chromosome. However, haESCs are prone to spontaneous diploidization through unknown mechanisms. Here, we report that a small molecule combination could restrain mouse haESCs from diploidization by impeding exit from naive pluripotency and by shortening the S-G2/M phases. Combined with 2i and PD166285, our chemical cocktail could maintain haESCs in the haploid state for at least five weeks without fluorescence-activated cell sorting (FACS) enrichment of haploid cells. Taken together, we established an effective chemical approach for long-term maintenance of haESCs, and highlighted that proper cell cycle progression was critical for the maintenance of haploid state.
收录类别	SCI
语种	英语
资助项目	Fundamental Research Funds for the Central Universities[2016QN81010]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000413048000033
出版者	NATURE PUBLISHING GROUP
WOS关键词	SELF-RENEWAL ; CYCLE PROGRESSION ; DNA METHYLATION ; IPS CELLS ; G1 PHASE ; IN-VITRO ; DIFFERENTIATION ; PLURIPOTENT ; GENERATION ; DERIVATION
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/9923
专题	生命科学与技术学院生命科学与技术学院_博士生免疫化学研究所_PI研究组_赵简组
通讯作者	Huang, Shichao; Shen, Li
作者单位	1.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol, Shanghai 200031, Peoples R China 2.Tongji Univ, Sch Life Sci & Technol, Shanghai Key Lab Signaling & Dis Res, Lab Receptor Based Biomed, Shanghai 200092, Peoples R China 3.Zhejiang Univ, Life Sci Inst, Hangzhou 310000, Zhejiang, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 5.Tongji Univ, Shanghai Matern & Infant Hosp 1, Sch Life Sci & Technol, Clin & Translat Res Ctr,Shanghai Key Lab Signalin, Shanghai 200092, Peoples R China 6.NIAMSD, Lab Muscle Stem Cells & Gene Regulat, NIH, Bethesda, MD 20892 USA
推荐引用方式 GB/T 7714	Li, Haisen,Guo, Ao,Xie, Zhenfei,et al. Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation[J]. SCIENTIFIC REPORTS,2017,7.
APA	Li, Haisen.,Guo, Ao.,Xie, Zhenfei.,Tu, Wanzhi.,Yu, Jiali.,...&Shen, Li.(2017).Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation.SCIENTIFIC REPORTS,7.
MLA	Li, Haisen,et al."Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation".SCIENTIFIC REPORTS 7(2017).