Key elements for designing and performing a CRISPR/Cas9-based genetic screen
Shang, Wanjing1,2,3; Wang, Fei1,2,3; Fan, Gaofeng1; Wang, Haopeng1
2017-09-20
Source PublicationJOURNAL OF GENETICS AND GENOMICS
ISSN1673-8527
Volume44Issue:9Pages:439-449
Status已发表
DOI10.1016/j.jgg.2017.09.005
AbstractReverse genetic screens are invaluable for uncovering gene functions, but are traditionally hampered by some technical limitations. Over the past few years, since the advent of the revolutionary CRISPR/Cas9 technology, its power in genome editing has been harnessed to overcome the traditional limitations in reverse genetic screens, with successes in various biological contexts. Here, we outline these CRISPR/Cas9-based screens, provide guidance on the design of effective screens and discuss the potential future directions of development of this field. Copyright (C) 2017, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.
KeywordCRISPR Cas9 Genetic screen
Indexed BySCI ; CSCD
Language英语
Funding ProjectNational Natural Science Foundation of China[31670919]
WOS Research AreaBiochemistry & Molecular Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Genetics & Heredity
WOS IDWOS:000413734200004
CSCD IDCSCD:6086882
PublisherSCIENCE PRESS
WOS KeywordWIDE CRISPR SCREEN ; ONE-STEP GENERATION ; GENOME-WIDE ; RNAI SCREEN ; MUTAGENESIS SCREEN ; IDENTIFIES GENES ; TRANSCRIPTIONAL ACTIVATION ; TARGETED MUTAGENESIS ; FUNCTIONAL GENOMICS ; CELLS
Original Document TypeReview
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://kms.shanghaitech.edu.cn/handle/2MSLDSTB/9902
Collection生命科学与技术学院
生命科学与技术学院_PI研究组_范高峰组
生命科学与技术学院_PI研究组_王皞鹏组
生命科学与技术学院_博士生
Corresponding AuthorFan, Gaofeng; Wang, Haopeng
Affiliation1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationSchool of Life Science and Technology
Corresponding Author AffilicationSchool of Life Science and Technology
First Signature AffilicationSchool of Life Science and Technology
Recommended Citation
GB/T 7714
Shang, Wanjing,Wang, Fei,Fan, Gaofeng,et al. Key elements for designing and performing a CRISPR/Cas9-based genetic screen[J]. JOURNAL OF GENETICS AND GENOMICS,2017,44(9):439-449.
APA Shang, Wanjing,Wang, Fei,Fan, Gaofeng,&Wang, Haopeng.(2017).Key elements for designing and performing a CRISPR/Cas9-based genetic screen.JOURNAL OF GENETICS AND GENOMICS,44(9),439-449.
MLA Shang, Wanjing,et al."Key elements for designing and performing a CRISPR/Cas9-based genetic screen".JOURNAL OF GENETICS AND GENOMICS 44.9(2017):439-449.
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