Key elements for designing and performing a CRISPR/Cas9-based genetic screen

doi:10.1016/j.jgg.2017.09.005

	Key elements for designing and performing a CRISPR/Cas9-based genetic screen
	Shang, Wanjing1,2,3 ; Wang, Fei1,2,3 ; Fan, Gaofeng1 ; Wang, Haopeng1
	2017-09-20
发表期刊	JOURNAL OF GENETICS AND GENOMICS
ISSN	1673-8527
卷号	44 期号:9 页码:439-449
发表状态	已发表
DOI	10.1016/j.jgg.2017.09.005
摘要	Reverse genetic screens are invaluable for uncovering gene functions, but are traditionally hampered by some technical limitations. Over the past few years, since the advent of the revolutionary CRISPR/Cas9 technology, its power in genome editing has been harnessed to overcome the traditional limitations in reverse genetic screens, with successes in various biological contexts. Here, we outline these CRISPR/Cas9-based screens, provide guidance on the design of effective screens and discuss the potential future directions of development of this field. Copyright (C) 2017, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.
关键词	CRISPR Cas9 Genetic screen
收录类别	SCI ; CSCD
资助项目	National Natural Science Foundation of China[31670919]
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:000413734200004
CSCD记录号	CSCD:6086882
出版者	SCIENCE PRESS
WOS关键词	WIDE CRISPR SCREEN ; ONE-STEP GENERATION ; GENOME-WIDE ; RNAI SCREEN ; MUTAGENESIS SCREEN ; IDENTIFIES GENES ; TRANSCRIPTIONAL ACTIVATION ; TARGETED MUTAGENESIS ; FUNCTIONAL GENOMICS ; CELLS
原始文献类型	Review
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/9902
专题	生命科学与技术学院生命科学与技术学院_PI研究组_范高峰组生命科学与技术学院_PI研究组_王皞鹏组生命科学与技术学院_博士生
共同第一作者	Wang, Fei
通讯作者	Fan, Gaofeng; Wang, Haopeng
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Shang, Wanjing,Wang, Fei,Fan, Gaofeng,et al. Key elements for designing and performing a CRISPR/Cas9-based genetic screen[J]. JOURNAL OF GENETICS AND GENOMICS,2017,44(9):439-449.
APA	Shang, Wanjing,Wang, Fei,Fan, Gaofeng,&Wang, Haopeng.(2017).Key elements for designing and performing a CRISPR/Cas9-based genetic screen.JOURNAL OF GENETICS AND GENOMICS,44(9),439-449.
MLA	Shang, Wanjing,et al."Key elements for designing and performing a CRISPR/Cas9-based genetic screen".JOURNAL OF GENETICS AND GENOMICS 44.9(2017):439-449.