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Molecular characteristics of early-stage female germ cells revealed by RNA sequencing of low-input cells and analysis of genome-wide DNA methylation | |
Ma, Binbin1; Lee, Tin-Lap2; Hu, Bian3,4; Li, Jing5; Li, Xiaoyong1; Zhao, Xiaodong6; Hou, Changliang1; Zhang, Chen1; He, Lin1; Huang, Xingxu3,4 | |
2019-04 | |
发表期刊 | DNA RESEARCH |
ISSN | 1340-2838 |
EISSN | 1756-1663 |
卷号 | 26期号:2页码:105-117 |
发表状态 | 已发表 |
DOI | 10.1093/dnares/dsy042 |
摘要 | High-throughput stage-specific transcriptomics provides an unbiased approach for understanding the process of cell development. Here, we report transcriptome analysis of primordial germ cell, female germline stem cell (FGSC), germinal vesicle and mature oocyte by performing RNA sequencing of freshly isolated cells in mice. As expected, these stages and gene-expression profiles are consistent with developmental timing. Analysis of genome-wide DNA methylation during female germline development was used for confirmation. By pathway analysis and blocking experiments, we demonstrate PI3K-AKT pathway is critical for FGSC maintenance. We also identify functional modules with hub genes and lncRNAs, which represent candidates for regulating FGSC self-renewal and differentiation. Remarkably, we note alternative splicing patterns change dramatically during female germline development, with the highest occurring in FGSCs. These findings are invaluable resource for dissecting the molecular pathways and processes into oogenesis and will be wider applications for other types of stem cell research. |
关键词 | female germ cells RNA sequencing transcriptomics DNA methylation development |
URL | 查看原文 |
收录类别 | SCI ; SCIE |
语种 | 英语 |
资助项目 | National Basic Research Program of China[2017YFA0504201] |
WOS研究方向 | Genetics & Heredity |
WOS类目 | Genetics & Heredity |
WOS记录号 | WOS:000493006800001 |
出版者 | OXFORD UNIV PRESS |
WOS关键词 | STEM-CELLS ; GENE ; DYNAMICS ; EXPRESSION ; OVARIES ; OOCYTES ; PROTEIN ; LINE ; CHROMOSOME ; EVENTS |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/80547 |
专题 | 生命科学与技术学院_PI研究组_黄行许组 |
通讯作者 | Lee, Tin-Lap; Li, Jing; Chen, Xuejin; Wu, Ji |
作者单位 | 1.Shanghai Jiao Tong Univ, Bio X Inst, Minist Educ, Key Lab Genet Dev & Neuropsychiat Disorders, Shanghai 200240, Peoples R China 2.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Reprod Dev & Endocrinol Program,Shatin, Hong Kong, Peoples R China 3.Nanjing Univ, Model Anim Res Ctr, MOE Key Lab Model Anim Dis Study, Nanjing 210061, Jiangsu, Peoples R China 4.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 5.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Dept Bioinformat & Biostat, Shanghai 200240, Peoples R China 6.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Shanghai 200240, Peoples R China 7.Shanghai Jiao Tong Univ, Dept Lab Anim Sci, Sch Med, Shanghai 200025, Peoples R China 8.Ningxia Med Univ, Minist Educ, Key Lab Fertil Preservat & Maintenance, Yinchuan 750004, Peoples R China |
推荐引用方式 GB/T 7714 | Ma, Binbin,Lee, Tin-Lap,Hu, Bian,et al. Molecular characteristics of early-stage female germ cells revealed by RNA sequencing of low-input cells and analysis of genome-wide DNA methylation[J]. DNA RESEARCH,2019,26(2):105-117. |
APA | Ma, Binbin.,Lee, Tin-Lap.,Hu, Bian.,Li, Jing.,Li, Xiaoyong.,...&Wu, Ji.(2019).Molecular characteristics of early-stage female germ cells revealed by RNA sequencing of low-input cells and analysis of genome-wide DNA methylation.DNA RESEARCH,26(2),105-117. |
MLA | Ma, Binbin,et al."Molecular characteristics of early-stage female germ cells revealed by RNA sequencing of low-input cells and analysis of genome-wide DNA methylation".DNA RESEARCH 26.2(2019):105-117. |
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