Molecular characteristics of early-stage female germ cells revealed by RNA sequencing of low-input cells and analysis of genome-wide DNA methylation
Ma, Binbin1; Lee, Tin-Lap2; Hu, Bian3,4; Li, Jing5; Li, Xiaoyong1; Zhao, Xiaodong6; Hou, Changliang1; Zhang, Chen1; He, Lin1; Huang, Xingxu3,4
2019-04
发表期刊DNA RESEARCH
ISSN1340-2838
EISSN1756-1663
卷号26期号:2页码:105-117
发表状态已发表
DOI10.1093/dnares/dsy042
摘要High-throughput stage-specific transcriptomics provides an unbiased approach for understanding the process of cell development. Here, we report transcriptome analysis of primordial germ cell, female germline stem cell (FGSC), germinal vesicle and mature oocyte by performing RNA sequencing of freshly isolated cells in mice. As expected, these stages and gene-expression profiles are consistent with developmental timing. Analysis of genome-wide DNA methylation during female germline development was used for confirmation. By pathway analysis and blocking experiments, we demonstrate PI3K-AKT pathway is critical for FGSC maintenance. We also identify functional modules with hub genes and lncRNAs, which represent candidates for regulating FGSC self-renewal and differentiation. Remarkably, we note alternative splicing patterns change dramatically during female germline development, with the highest occurring in FGSCs. These findings are invaluable resource for dissecting the molecular pathways and processes into oogenesis and will be wider applications for other types of stem cell research.
关键词female germ cells RNA sequencing transcriptomics DNA methylation development
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收录类别SCI ; SCIE
语种英语
资助项目National Basic Research Program of China[2017YFA0504201]
WOS研究方向Genetics & Heredity
WOS类目Genetics & Heredity
WOS记录号WOS:000493006800001
出版者OXFORD UNIV PRESS
WOS关键词STEM-CELLS ; GENE ; DYNAMICS ; EXPRESSION ; OVARIES ; OOCYTES ; PROTEIN ; LINE ; CHROMOSOME ; EVENTS
原始文献类型Article
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/80547
专题生命科学与技术学院_PI研究组_黄行许组
通讯作者Lee, Tin-Lap; Li, Jing; Chen, Xuejin; Wu, Ji
作者单位1.Shanghai Jiao Tong Univ, Bio X Inst, Minist Educ, Key Lab Genet Dev & Neuropsychiat Disorders, Shanghai 200240, Peoples R China
2.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Reprod Dev & Endocrinol Program,Shatin, Hong Kong, Peoples R China
3.Nanjing Univ, Model Anim Res Ctr, MOE Key Lab Model Anim Dis Study, Nanjing 210061, Jiangsu, Peoples R China
4.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
5.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Dept Bioinformat & Biostat, Shanghai 200240, Peoples R China
6.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Shanghai 200240, Peoples R China
7.Shanghai Jiao Tong Univ, Dept Lab Anim Sci, Sch Med, Shanghai 200025, Peoples R China
8.Ningxia Med Univ, Minist Educ, Key Lab Fertil Preservat & Maintenance, Yinchuan 750004, Peoples R China
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Ma, Binbin,Lee, Tin-Lap,Hu, Bian,et al. Molecular characteristics of early-stage female germ cells revealed by RNA sequencing of low-input cells and analysis of genome-wide DNA methylation[J]. DNA RESEARCH,2019,26(2):105-117.
APA Ma, Binbin.,Lee, Tin-Lap.,Hu, Bian.,Li, Jing.,Li, Xiaoyong.,...&Wu, Ji.(2019).Molecular characteristics of early-stage female germ cells revealed by RNA sequencing of low-input cells and analysis of genome-wide DNA methylation.DNA RESEARCH,26(2),105-117.
MLA Ma, Binbin,et al."Molecular characteristics of early-stage female germ cells revealed by RNA sequencing of low-input cells and analysis of genome-wide DNA methylation".DNA RESEARCH 26.2(2019):105-117.
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