Internalization Characterization of Si Nanorod with Camouflaged Cell Membrane Proteins Reveals ATXN2 as a Negative Regulator
Lu, Yi1,2,3; Dai, Jing3,4,5; Kong, Na3; Liu, Jianghuai1,2; Gong, Jinkang3; Yao, Yuan3
2019-08
发表期刊CELLS
卷号8期号:8
发表状态已发表
DOI10.3390/cells8080931
摘要The fabrication of shape-controlled nanocarriers is critical for efficient delivery of biomolecules across the cell membrane. Surface coating of the nanocarrier can improve internalization efficiency. Here, we developed a facile method of silicon nanorod fabrication leading to a controlled size and shape. We then systematically evaluated five surface modifications with membrane proteins from different cancer cell lines including MCF7, MD231, Hela, Panc-PDX, and Panc-1. We demonstrated that silicon nanorods coated with either a homolytic or heterolytic membrane protein coating have significantly improved internalization efficiency as compared with uncoated Si nanorods. To elucidate the molecular mechanism of the improved efficiency associated with a modified coating, we analyzed the coating membrane proteins derived from five cell lines with proteomics and identified 601 proteins shared by different cell sources. These proteins may function as cell-substrate adhesion molecules that contribute to the enhanced internalization. We also tested the internalization efficiency of nanorods with different coatings in each of the five cell lines to determine the influencing factors from target cells. We found that the internalization efficiency varied among different target cells, and the ranking of the average efficiency was as follows: Hela > Panc-PDX > MD231 > MCF7 > Panc-1. The bioinformatics analysis suggested that the low internalization efficiency in Panc-1 cells might be associated with the upregulation of ATXN2, which is a negative regulator of endocytosis. We further demonstrated that ATXN2 knockdown with specific siRNA significantly improved nanorod internalization efficiency in Panc-1 cells suggesting that ATXN2 can be a reference for efficiency prediction of nanoparticle delivery to tumor cells. Thus, we studied the effect of different cancer cell membrane proteins on nanorod uptake efficiencies. These results can improve nanorod internalization to cancer cells, including a fundamental understanding of the internalization efficiency of cancer cells.
关键词silicon nanorod cell membrane proteins cancer internalization efficiency ATXN2
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收录类别SCI ; SCIE
语种英语
资助项目ShanghaiTech University[F-0201-13-001]
WOS研究方向Cell Biology
WOS类目Cell Biology
WOS记录号WOS:000484537500164
出版者MDPI
WOS关键词BIOMIMETIC NANOPARTICLES ; POLYMERIC NANOPARTICLES ; MELANOTRANSFERRIN P97 ; HIGHLY EFFICIENT ; GRAPHENE OXIDE ; CANCER ; SHAPE ; SILICON ; RECEPTOR ; ENDOCYTOSIS
原始文献类型Article
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/79726
专题物质科学与技术学院_PI研究组_龚晋慷组
附属学校、幼儿园筹建部
物质科学与技术学院_博士生
通讯作者Yao, Yuan
作者单位1.Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Model Anim Res Ctr, Nanjing 210061, Jiangsu, Peoples R China
2.Nanjing Univ, Minist Educ, Model Anim Res Ctr, Key Lab Model Anim Dis Study, Nanjing 210061, Jiangsu, Peoples R China
3.ShanghaiTech Univ, Sch Phys Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Ceram, Shanghai 200050, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位物质科学与技术学院
通讯作者单位物质科学与技术学院
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GB/T 7714
Lu, Yi,Dai, Jing,Kong, Na,et al. Internalization Characterization of Si Nanorod with Camouflaged Cell Membrane Proteins Reveals ATXN2 as a Negative Regulator[J]. CELLS,2019,8(8).
APA Lu, Yi,Dai, Jing,Kong, Na,Liu, Jianghuai,Gong, Jinkang,&Yao, Yuan.(2019).Internalization Characterization of Si Nanorod with Camouflaged Cell Membrane Proteins Reveals ATXN2 as a Negative Regulator.CELLS,8(8).
MLA Lu, Yi,et al."Internalization Characterization of Si Nanorod with Camouflaged Cell Membrane Proteins Reveals ATXN2 as a Negative Regulator".CELLS 8.8(2019).
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