Exploiting mammalian low-complexity domains for liquid-liquid phase separation-driven underwater adhesive coatings

doi:10.1126/sciadv.aax3155

	Exploiting mammalian low-complexity domains for liquid-liquid phase separation-driven underwater adhesive coatings
	Cui, Mengkui1,2,3 ; Wang, Xinyu1,2,4 ; An, Bolin1 ; Zhang, Chen1 ; Gui, Xinrui 2,5; Li, Ke1 ; Li, Yingfeng1,2,4 ; Ge, Peng 6; Zhang, Junhu 6; Liu, Cong 5; Zhong, Chao1
	2019-08
发表期刊	SCIENCE ADVANCES
ISSN	2375-2548
卷号	5 期号:8
发表状态	已发表
DOI	10.1126/sciadv.aax3155
摘要	Many biological materials form via liquid-liquid phase separation (LLPS), followed by maturation into a solid-like state. Here, using a biologically inspired assembly mechanism designed to recapitulate these sequential assemblies, we develop ultrastrong underwater adhesives made from engineered proteins containing mammalian low-complexity (LC) domains. We show that LC domain-mediated LLPS and maturation substantially promotes the wetting, adsorption, priming, and formation of dense, uniform amyloid nanofiber coatings on diverse surfaces (e.g., Teflon), and even penetrating difficult-to-access locations such as the interiors of microfluidic devices. Notably, these coatings can be deposited on substrates over a broad range of pH values (3 to 11) and salt concentrations (up to 1 M NaCl) and exhibit strong underwater adhesion performance. Beyond demonstrating the utility of mammalian LC domains for driving LLPS in soft materials applications, our study illustrates a powerful example of how combining LLPS with subsequent maturation steps can be harnessed for engineering protein-based materials.
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收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0403]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000482759500023
出版者	AMER ASSOC ADVANCEMENT SCIENCE
EI主题词	Adhesives ; Beryllium compounds ; Biological materials ; Coatings ; Liquids ; Mammals ; Proteins ; Sodium chloride
WOS关键词	CELL-FREE FORMATION ; GRANULES ; COACERVATION ; AMYLOIDS ; DROPLETS
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/67955
专题	物质科学与技术学院_博士生物质科学与技术学院_PI研究组_钟超组
通讯作者	Zhong, Chao
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, Mat & Phys Biol Div, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Ceram, Shanghai 200050, Peoples R China 5.Chinese Acad Sci, Interdisciplinary Res Ctr Biol & Chem, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 6.Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, Changchun 130012, Jilin, Peoples R China
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Cui, Mengkui,Wang, Xinyu,An, Bolin,et al. Exploiting mammalian low-complexity domains for liquid-liquid phase separation-driven underwater adhesive coatings[J]. SCIENCE ADVANCES,2019,5(8).
APA	Cui, Mengkui.,Wang, Xinyu.,An, Bolin.,Zhang, Chen.,Gui, Xinrui.,...&Zhong, Chao.(2019).Exploiting mammalian low-complexity domains for liquid-liquid phase separation-driven underwater adhesive coatings.SCIENCE ADVANCES,5(8).
MLA	Cui, Mengkui,et al."Exploiting mammalian low-complexity domains for liquid-liquid phase separation-driven underwater adhesive coatings".SCIENCE ADVANCES 5.8(2019).