Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans

doi:10.1038/s41564-019-0445-2

	Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans
	He, Tao 1; Wang, Ran 1; Liu, Dejun 2; Walsh, Timothy R.2,3; Zhang, Rong 4; Lv, Yuan 5; Ke, Yuebin 6; Ji, Quanjiang7 ; Wei, Ruicheng 1; Liu, Zhihai 2; Shen, Yingbo 2; Wang, Gang 1; Sun, Lichang 1; Lei, Lei 2; Lv, Ziquan 6; Li, Yun 5; Pang, Maoda 1; Wang, Liyuan 5; Sun, Qiaoling 4; Fu, Yulin 2; Song, Huangwei 2; Hao, Yuxin 2; Shen, Zhangqi 2; Wang, Shaolin 2; Chen, Gongxiang 4; Wu, Congming 2; Shen, Jianzhong 2,8; Wang, Yang 2,8
	2019-09
发表期刊	NATURE MICROBIOLOGY
ISSN	2058-5276
卷号	4 期号:9 页码:1450-1456
发表状态	已发表
DOI	10.1038/s41564-019-0445-2
摘要	Tigecycline is a last-resort antibiotic that is used to treat severe infections caused by extensively drug-resistant bacteria.tet(X) has been shown to encode a flavin-dependent monooxygenase that modifies tigecycline(1,2). Here, we report two unique mobile tigecycline-resistance genes, tet(X3) and tet(X4), in numerous Enterobacteriaceae and Acinetobacter that were isolated from animals, meat for consumption and humans. Tet(X3) and Tet(X4) inactivate all tetracyclines, including tigecycline and the newly FDA-approved eravacycline and omadacycline. Both tet(X3) and tet(X4) increase (by 64-128-fold) the tigecycline minimal inhibitory concentration values for Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii. In addition, both Tet(X3) (A. baumannii) and Tet(X4) (E. coli) significantly compromise tigecycline in in vivo infection models. Both tet(X3) and tet(X4) are adjacent to insertion sequence ISVsa3 on their respective conjugative plasmids and confer a mild fitness cost (relative fitness of >0.704). Database mining and retrospective screening analyses confirm that tet(X3) and tet(X4) are globally present in clinical bacteria-even in the same bacteria as bla(NDM-1), resulting in resistance to both tigecycline and carbapenems. Our findings suggest that both the surveillance of tet(X) variants in clinical and animal sectors and the use of tetracyclines in food production require urgent global attention.
收录类别	SCI ; SCIE
语种	英语
资助项目	China Agriculture Research System[CARS-36]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000482228400007
出版者	NATURE PUBLISHING GROUP
WOS关键词	TETRACYCLINE RESISTANCE ; ACINETOBACTER-BAUMANNII ; ESCHERICHIA-COLI ; EFFICACY ; SAFETY ; INFECTIONS ; TETX
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/66396
专题	物质科学与技术学院_PI研究组_季泉江组
通讯作者	Shen, Jianzhong; Wang, Yang
作者单位	1.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr,Minist Agr & Rural Affair, Key Lab Control Technol & Stand Agroprod Safety &, Jiangsu Key Lab Food Qual & Safety,State Key Lab, Nanjing, Jiangsu, Peoples R China 2.China Agr Univ, Coll Vet Med, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing, Peoples R China 3.Inst Infect & Immun, Dept Med Microbiol & Infect Dis, Cardiff, S Glam, Wales 4.Zhejiang Univ, Affiliated Hosp 2, Hangzhou, Zhejiang, Peoples R China 5.Peking Univ, Hosp 1, Inst Clin Pharmacol, Beijing, Peoples R China 6.Shenzhen Ctr Dis Control & Prevent, Key Lab Mol Epidemiol Shenzhen, Shenzhen, Peoples R China 7.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai, Peoples R China 8.China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food, Beijing, Peoples R China
推荐引用方式 GB/T 7714	He, Tao,Wang, Ran,Liu, Dejun,et al. Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans[J]. NATURE MICROBIOLOGY,2019,4(9):1450-1456.
APA	He, Tao.,Wang, Ran.,Liu, Dejun.,Walsh, Timothy R..,Zhang, Rong.,...&Wang, Yang.(2019).Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans.NATURE MICROBIOLOGY,4(9),1450-1456.
MLA	He, Tao,et al."Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans".NATURE MICROBIOLOGY 4.9(2019):1450-1456.