Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins
Xue, Dongxiang1,2,3,4; Xu, Tiandan1,3,4,5; Wang, Huixia1,3,4,5; Wu, Meng1,3,4,5; Yuan, Ya1; Wang, Wei6; Tan, Qiwen1; Zhao, Fei1; Zhou, Fang1,2,3,4; Hu, Tao1,3,4,5; Jiang, Zhongxing6; Liu, Zhi-Jie1,4; Zhao, Suwen1,4; Liu, Dongsheng1; Wuthrich, Kurt1,4; Tao, Houchao1
2019-08-13
Source PublicationCHEMISTRY-A EUROPEAN JOURNAL
ISSN0947-6539
Status已发表
DOI10.1002/chem.201903190
AbstractDisulfide-containing detergents (DCDs) are introduced, which contain a disulfide bond in the hydrophobic tail. DCDs form smaller micelles than corresponding detergents with linear hydrocarbon chains, while providing good solubilization and reconstitution of membrane proteins. The use of this new class of detergents in structural biology is illustrated with solution NMR spectra of the human G protein-coupled receptor A(2A)AR, which is an alpha-helical protein, and the beta-barrel protein OmpX from E. coli.
Keyworddetergents disulfides membrane proteins micelle size NMR spectroscopy
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[21672147] ; National Natural Science Foundation of China[31670733]
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000481113100001
PublisherWILEY-V C H VERLAG GMBH
EI KeywordsCovalent bonds ; Detergents ; Escherichia coli ; Micelles ; Nuclear magnetic resonance spectroscopy ; Soaps (detergents) ; Sulfur compounds
WOS KeywordCRYSTAL-STRUCTURE ; RECEPTOR ; NMR ; AMPHIPHILES ; STABILITY ; MICELLES ; COMPLEX ; ACIDS ; BILE
Original Document TypeArticle ; Early Access
Citation statistics
Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://kms.shanghaitech.edu.cn/handle/2MSLDSTB/64583
Collection生命科学与技术学院_博士生
iHuman研究所_特聘教授组_Kurt Wuthrich组
iHuman研究所_公共科研平台_克隆平台
iHuman研究所_PI研究组_刘志杰组
iHuman研究所_PI研究组_陶厚朝组
iHuman研究所_PI研究组_赵素文组
生命科学与技术学院_硕士生
Corresponding AuthorWuthrich, Kurt; Tao, Houchao
Affiliation1.ShanghaiTech Univ, iHuman Inst, Ren Bldg,393 Middle Huaxia Rd, Shanghai 201210, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China
3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
5.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
6.Wuhan Univ, Hubei Prov Engn & Technol Res Ctr Fluorinated Pha, Sch Pharmaceut Sci, Wuhan 430071, Hubei, Peoples R China
First Author AffilicationiHuman Institute;  School of Life Science and Technology
Corresponding Author AffilicationiHuman Institute;  School of Life Science and Technology
First Signature AffilicationiHuman Institute
Recommended Citation
GB/T 7714
Xue, Dongxiang,Xu, Tiandan,Wang, Huixia,et al. Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins[J]. CHEMISTRY-A EUROPEAN JOURNAL,2019.
APA Xue, Dongxiang.,Xu, Tiandan.,Wang, Huixia.,Wu, Meng.,Yuan, Ya.,...&Tao, Houchao.(2019).Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins.CHEMISTRY-A EUROPEAN JOURNAL.
MLA Xue, Dongxiang,et al."Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins".CHEMISTRY-A EUROPEAN JOURNAL (2019).
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