Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins

doi:10.1002/chem.201903190

	Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins
	Xue, Dongxiang1,2,3,4 ; Xu, Tiandan1,3,4,5 ; Wang, Huixia1,3,4,5 ; Wu, Meng1,3,4,5 ; Yuan, Ya1 ; Wang, Wei 6; Tan, Qiwen1 ; Zhao, Fei1 ; Zhou, Fang 1,2,3,4; Hu, Tao1,3,4,5 ; Jiang, Zhongxing 6; Liu, Zhi-Jie1,4 ; Zhao, Suwen1,4 ; Liu, Dongsheng1 ; Wuthrich, Kurt1,4 ; Tao, Houchao1
	2019-08-13
发表期刊	CHEMISTRY-A EUROPEAN JOURNAL
ISSN	0947-6539
发表状态	已发表
DOI	10.1002/chem.201903190
摘要	Disulfide-containing detergents (DCDs) are introduced, which contain a disulfide bond in the hydrophobic tail. DCDs form smaller micelles than corresponding detergents with linear hydrocarbon chains, while providing good solubilization and reconstitution of membrane proteins. The use of this new class of detergents in structural biology is illustrated with solution NMR spectra of the human G protein-coupled receptor A(2A)AR, which is an alpha-helical protein, and the beta-barrel protein OmpX from E. coli.
关键词	detergents disulfides membrane proteins micelle size NMR spectroscopy
收录类别	SCI ; SCIE ; EI ; IC
语种	英语
资助项目	National Natural Science Foundation of China[21672147] ; National Natural Science Foundation of China[31670733]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000481113100001
出版者	WILEY-V C H VERLAG GMBH
EI主题词	Covalent bonds ; Detergents ; Escherichia coli ; Micelles ; Nuclear magnetic resonance spectroscopy ; Soaps (detergents) ; Sulfur compounds
WOS关键词	CRYSTAL-STRUCTURE ; RECEPTOR ; NMR ; AMPHIPHILES ; STABILITY ; MICELLES ; COMPLEX ; ACIDS ; BILE
原始文献类型	Article ; Early Access
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/64583
专题	生命科学与技术学院_博士生 iHuman研究所 iHuman研究所_特聘教授组_Kurt Wuthrich组 iHuman研究所_公共科研平台_克隆平台 iHuman研究所_PI研究组_刘志杰组 iHuman研究所_PI研究组_陶厚朝组 iHuman研究所_PI研究组_赵素文组生命科学与技术学院_硕士生 iHuman研究所_PI研究组_华甜组
通讯作者	Wuthrich, Kurt; Tao, Houchao
作者单位	1.ShanghaiTech Univ, iHuman Inst, Ren Bldg,393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 5.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 6.Wuhan Univ, Hubei Prov Engn & Technol Res Ctr Fluorinated Pha, Sch Pharmaceut Sci, Wuhan 430071, Hubei, Peoples R China
第一作者单位	iHuman研究所; 生命科学与技术学院
通讯作者单位	iHuman研究所; 生命科学与技术学院
第一作者的第一单位	iHuman研究所
推荐引用方式 GB/T 7714	Xue, Dongxiang,Xu, Tiandan,Wang, Huixia,et al. Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins[J]. CHEMISTRY-A EUROPEAN JOURNAL,2019.
APA	Xue, Dongxiang.,Xu, Tiandan.,Wang, Huixia.,Wu, Meng.,Yuan, Ya.,...&Tao, Houchao.(2019).Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins.CHEMISTRY-A EUROPEAN JOURNAL.
MLA	Xue, Dongxiang,et al."Disulfide-Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins".CHEMISTRY-A EUROPEAN JOURNAL (2019).