An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast

doi:10.1007/s10529-019-02710-5

KMS > 免疫化学研究所 > 特聘教授组 > Richard A. Lerner组

	An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast
	Jia, Yanrong 1; Ren, Ping2 ; Duan, Shixin 1; Zeng, Pei 1; Xie, Debao 1; Zeng, Fanli 1
	2019-09
发表期刊	BIOTECHNOLOGY LETTERS (IF:2.0[JCR-2023],2.4[5-Year])
ISSN	0141-5492
卷号	41 期号:8-9 页码:1067-1076
发表状态	已发表
DOI	10.1007/s10529-019-02710-5
摘要	ObjectivesEstablish a method to restrict unexpected fragments including stop codons in scFv library and generate a thermo resistant strain for screening of thermal stable scFv sequences.ResultsHere, we have constructed a T2A-Leu2 system for selection of yeast surface display libraries that blocks amplification of stop codon plasmids within the library, thereby increasing the quality of the library and efficiency of the selection screen. Also, we generated a temperature-resistant yeast strain, TR1, and validated its combined use with T2A-Leu2 for efficient screening. Thus, we developed a general approach for a fast and efficient screening of scFv libraries using a ribosomal skipping system and thermo-resistant yeast.ConclusionsThe method highlights the utility of the T2A-Leu2-based ribosomal skipping strategy for increasing the quality of the input library for selection, along with an optimized selection protocol based on thermo-resistant yeast cells.
关键词	Yeast surface display scFv antibody Ribosomal skipping T2A Antibody screen
收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	National Natural Science Foundation of China[31801039]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000477931700019
出版者	SPRINGER
WOS关键词	SURFACE DISPLAY ; CLEAVAGE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/61138
专题	免疫化学研究所_特聘教授组_抗体设计学实验室
通讯作者	Zeng, Fanli
作者单位	1.Hebei Agr Univ, Coll Life Sci, Baoding 071001, Hebei, Peoples R China 2.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Jia, Yanrong,Ren, Ping,Duan, Shixin,et al. An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast[J]. BIOTECHNOLOGY LETTERS,2019,41(8-9):1067-1076.
APA	Jia, Yanrong,Ren, Ping,Duan, Shixin,Zeng, Pei,Xie, Debao,&Zeng, Fanli.(2019).An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast.BIOTECHNOLOGY LETTERS,41(8-9),1067-1076.
MLA	Jia, Yanrong,et al."An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast".BIOTECHNOLOGY LETTERS 41.8-9(2019):1067-1076.