Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome

doi:10.7554/eLife.45057

	Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome
	Song, Xiyong 1,2,7; Shan, Hong3 ; Zhu, Yanping 2; Hu, Shunlin 4; Xue, Ling 4; Chen, Yong 2,7; Ding, Wei 6; Niu, Tongxin 6; Gu, Jian 4; Ouyang, Songying 5,8; Shen, Qing-Tao3 ; Liu, Zhi-Jie1,2,3
	2019-07-10
发表期刊	ELIFE (IF:6.4[JCR-2023],7.2[5-Year])
ISSN	2050-084X
卷号	8
发表状态	已发表
DOI	10.7554/eLife.45057
摘要	Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 angstrom resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode. This tightly packed structure functions as a seed for the assembly of a nucleocapsid from both directions, facilitating the growth of double-headed filaments with two separate RNA strings inside. Disruption of this structure by mutations in its loop interface yielded a single-headed unfunctional filament.
收录类别	SCI ; SCIE
语种	英语
资助项目	National Key R&D Programme of China[2017YFA0504800]
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:000478859700001
出版者	ELIFE SCIENCES PUBLICATIONS LTD
WOS关键词	VESICULAR STOMATITIS-VIRUS ; N-TERMINAL REGION ; CRYO-EM STRUCTURE ; MEASLES-VIRUS ; NUCLEOCAPSID PROTEIN ; CRYSTAL-STRUCTURE ; RNA-SYNTHESIS ; MATRIX PROTEIN ; RAD51 PARALOGS ; COMPLEX
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/61137
专题	iHuman研究所_PI研究组_沈庆涛组 iHuman研究所_PI研究组_刘志杰组
通讯作者	Ouyang, Songying; Shen, Qing-Tao; Liu, Zhi-Jie
作者单位	1.Kunming Med Univ, Inst Mol & Clin Med, Kunming, Yunnan, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing, Peoples R China 3.ShanghaiTech Univ, iHuman Inst, Shanghai, Peoples R China 4.Yangzhou Univ, Coll Vet Med, Yangzhou, Jiangsu, Peoples R China 5.Fujian Normal Univ, Coll Life Sci, Minist Educ,Key Lab OptoElect Sci & Technol Med, Key Lab Innate Immune Biol Fujian Prov,Biomed Res, Fuzhou, Fujian, Peoples R China 6.Chinese Acad Sci, Ctr Biol Imaging, Inst Biophys, Beijing, Peoples R China 7.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 8.Fujian Normal Univ, Coll Life Sci, Prov Univ Key Lab Cellular Stress Response & Meta, Fuzhou, Fujian, Peoples R China
通讯作者单位	iHuman研究所
推荐引用方式 GB/T 7714	Song, Xiyong,Shan, Hong,Zhu, Yanping,et al. Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome[J]. ELIFE,2019,8.
APA	Song, Xiyong.,Shan, Hong.,Zhu, Yanping.,Hu, Shunlin.,Xue, Ling.,...&Liu, Zhi-Jie.(2019).Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome.ELIFE,8.
MLA	Song, Xiyong,et al."Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome".ELIFE 8(2019).