Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells

doi:10.3390/ijms20112753

	Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells
	Li, Xin 1,2; Diao, Min3,4 ; Zhang, Yanan 5; Chen, Guanlin 6; Huang, Shanjin 3; Chen, Naizhi 1
	2019-06
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (IF:4.9[JCR-2023],5.6[5-Year])
ISSN	1422-0067
卷号	20 期号:11
发表状态	已发表
DOI	10.3390/ijms20112753
摘要	The actin cytoskeleton is involved in regulating stomatal movement, which forms distinct actin arrays within guard cells of stomata with different apertures. How those actin arrays are formed and maintained remains largely unexplored. Elucidation of the dynamic behavior of differently oriented actin filaments in guard cells will enhance our understanding in this regard. Here, we initially developed a program called guard cell microfilament analyzer' (GCMA) that enables the selection of individual actin filaments and analysis of their orientations semiautomatically in guard cells. We next traced the dynamics of individual actin filaments and performed careful quantification in open and closed stomata. We found that de novo nucleation of actin filaments occurs at both dorsal and ventral sides of guard cells from open and closed stomata. Interestingly, most of the nucleated actin filaments elongate radially and longitudinally in open and closed stomata, respectively. Strikingly, radial filaments tend to form bundles whereas longitudinal filaments tend to be removed by severing and depolymerization in open stomata. By contrast, longitudinal filaments tend to form bundles that are severed less frequently in closed stomata. These observations provide insights into the formation and maintenance of distinct actin arrays in guard cells in stomata of different apertures.
关键词	guard cell microfilament angle (GCMA) individual actin filaments different orientations actin dynamics technical advance Arabidopsis
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31671398]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000472634100147
出版者	MDPI
WOS关键词	STOMATAL MOVEMENT ; MICROTUBULE ORGANIZATION ; ABSCISIC-ACID ; REORGANIZATION ; TURNOVER ; CLOSURE ; FUSION ; GROWTH ; ARRAY
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/61114
专题	iHuman研究所_公共科研平台_超分辨生物成像平台
通讯作者	Huang, Shanjin; Chen, Naizhi
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China 4.Shanghai Tech Univ, iHuman Inst, Shanghai 201210, Peoples R China 5.OLYMPUS CHINA CO LTD, Beijing 100027, Peoples R China 6.Baidu Online Network Technol Beijing CO LTD, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Li, Xin,Diao, Min,Zhang, Yanan,et al. Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(11).
APA	Li, Xin,Diao, Min,Zhang, Yanan,Chen, Guanlin,Huang, Shanjin,&Chen, Naizhi.(2019).Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(11).
MLA	Li, Xin,et al."Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.11(2019).