Citrate-assisted efficient local delivery of naked oligonucleotide into live mouse brain cells

doi:10.1111/cpr.12622

	Citrate-assisted efficient local delivery of naked oligonucleotide into live mouse brain cells
	Zhou, Haibin 1,2,3,4; Zhang, Shouhua1,2,3,4 ; Lv, Fei3,4,5,6 ; Sun, Wenzhi4,5,7 ; Wang, Lihua 1,2; Fan, Chunhai 1,2; Li, Jiang 1,2; Hu, Ji4
	2019-07
发表期刊	CELL PROLIFERATION (IF:5.9[JCR-2023],7.3[5-Year])
ISSN	0960-7722
卷号	52 期号:4
发表状态	已发表
DOI	10.1111/cpr.12622
摘要	Objectives Synthetic oligonucleotides have shown promise in brain imaging. However, delivery of oligonucleotides into live brain cells remains challenging. In this study, we aim to develop a facile yet efficient strategy for local delivery of oligodeoxynucleotide (ODN) to neural cells in live adult mouse brain. Materials and methods A fluorescence-labelled ODN was diluted with sodium citrate buffer (100 mmol/L, pH = 3). One microlitre of the mixture was injected into a live adult mouse brain. Six hours later, we sacrificed the mouse and prepared brain slices for microscopic imaging. Results We find that the use of sodium citrate buffer in the one-shot local delivery can improve the diffusion and cell entry efficiency of the unmodified ODN for dozens of times. Only 1 pmol ODN leads to hundreds of positively transferred brain cells. We reason that this promotion is due to the local acidic condition created by the citrate buffer, which leads to the protonation of the ODN and some membrane proteins, thus reduces the Coulomb repulsion between the ODN and the cell membrane. Based on this strategy, we demonstrate fluorescent microscopic imaging of brain cells in different brain regions including striatum, cortex, hippocampus and midbrain. Conclusions The citrate buffer can be used as an adjuvant for facile and effective local injection delivery of ODNs, which may provide a new tool for brain imaging.
关键词	neuronal transfection ssDNA stereotaxic injection vector-free
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[21775157] ; National Natural Science Foundation of China[31671086] ; National Natural Science Foundation of China[61890950] ; National Natural Science Foundation of China[61890951]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000477913100003
出版者	WILEY
WOS关键词	GOLD NANOPARTICLES ; DNA ; PROLIFERATION ; DISEASE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/61050
专题	生命科学与技术学院_硕士生生命科学与技术学院_PI研究组_胡霁组 iHuman研究所_PI研究组_孙文智组
通讯作者	Li, Jiang; Hu, Ji
作者单位	1.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China 2.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai 201800, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 5.ShanghaiTech Univ, IHuman Inst, Shanghai, Peoples R China 6.Chinese Acad Sci, Inst Neurosci, Shanghai, Peoples R China 7.Chinese Inst Brain Res, Beijing, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhou, Haibin,Zhang, Shouhua,Lv, Fei,et al. Citrate-assisted efficient local delivery of naked oligonucleotide into live mouse brain cells[J]. CELL PROLIFERATION,2019,52(4).
APA	Zhou, Haibin.,Zhang, Shouhua.,Lv, Fei.,Sun, Wenzhi.,Wang, Lihua.,...&Hu, Ji.(2019).Citrate-assisted efficient local delivery of naked oligonucleotide into live mouse brain cells.CELL PROLIFERATION,52(4).
MLA	Zhou, Haibin,et al."Citrate-assisted efficient local delivery of naked oligonucleotide into live mouse brain cells".CELL PROLIFERATION 52.4(2019).