ShanghaiTech University Knowledge Management System
Metabolic regulation of cytoskeleton functions by HDAC6-catalyzed α-tubulin lactylation | |
2024-09-27 | |
发表期刊 | NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year]) |
ISSN | 2041-1723 |
EISSN | 2041-1723 |
卷号 | 15期号:1 |
发表状态 | 已发表 |
DOI | 10.1038/s41467-024-52729-0 |
摘要 | Posttranslational modifications (PTMs) of tubulin, termed the “tubulin code”, play important roles in regulating microtubule functions within subcellular compartments for specialized cellular activities. While numerous tubulin PTMs have been identified, a comprehensive understanding of the complete repertoire is still underway. In this study, we report that α-tubulin lactylation is catalyzed by HDAC6 by using lactate to increase microtubule dynamics in neurons. We identify lactylation on lysine 40 of α-tubulin in the soluble tubulin dimers. Notably, lactylated α-tubulin enhances microtubule dynamics and facilitates neurite outgrowth and branching in cultured hippocampal neurons. Moreover, we discover an unexpected function of HDAC6, acting as the primary lactyltransferase to catalyze α-tubulin lactylation. HDAC6-catalyzed lactylation is a reversible process, dependent on lactate concentrations. Intracellular lactate concentration triggers HDAC6 to lactylate α-tubulin, a process dependent on its deacetylase activity. Additionally, the lactyltransferase activity may be conserved in HDAC family proteins. Our study reveals the primary role of HDAC6 in regulating α-tubulin lactylation, establishing a link between cell metabolism and cytoskeleton functions. |
关键词 | Microtubule PTMs Lactylation HDAC6 Microtubule dynamic instabilit |
学科门类 | 理学 ; 理学::生物学 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/527166 |
专题 | 生命科学与技术学院_博士生 生命科学与技术学院_PI研究组_李磊组 生命科学与技术学院_硕士生 生命科学与技术学院_PI研究组_孙亚东组 |
共同第一作者 | Zhe Xu |
通讯作者 | Yadong Sun |
作者单位 | 1.ShanghaiTech University 2.Fudan Univ, Huadong Hosp, Dept Pain Management, Shanghai, Peoples R China 3.Shanghai Clin Res & Trial Ctr, Shanghai, Peoples R China 4.Army Medical University |
第一作者单位 | 上海科技大学 |
通讯作者单位 | 上海科技大学 |
第一作者的第一单位 | 上海科技大学 |
推荐引用方式 GB/T 7714 | Shuangshuang Sun,Zhe Xu,Liying He,et al. Metabolic regulation of cytoskeleton functions by HDAC6-catalyzed α-tubulin lactylation[J]. NATURE COMMUNICATIONS,2024,15(1). |
APA | Shuangshuang Sun.,Zhe Xu.,Liying He.,Yihui Shen.,Yuqing Yan.,...&Lei Li.(2024).Metabolic regulation of cytoskeleton functions by HDAC6-catalyzed α-tubulin lactylation.NATURE COMMUNICATIONS,15(1). |
MLA | Shuangshuang Sun,et al."Metabolic regulation of cytoskeleton functions by HDAC6-catalyzed α-tubulin lactylation".NATURE COMMUNICATIONS 15.1(2024). |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 |
修改评论
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。