ShanghaiTech University Knowledge Management System
| A Palette of Bridged Bicycle-Strengthened Fluorophores | |
| 2024-12-22 | |
| 状态 | 已发表 |
| 摘要 | Organic fluorophores are the keystone of advanced biological imaging. The vast chemical space of fluorophores has been extensively explored in seek of molecules with ideal properties. However, within the current molecular constraints, there appears to be a trade-off between high brightness, robust photostability, and tunable biochemical properties. Herein we report a general strategy to systematically boost the performance of donor-acceptor-type fluorophores by leveraging SO2 and O-substituted azabicyclo[3.2.1] octane auxochromes. These bicyclic heterocycles give rise to a collection of 'Bridged' dyes (BD) spanning the UV and visible range with top-notch quantum efficiencies, enhanced water solubility, and tunable cell-permeability. Notably, these azabicyclic fluorophores showed remarkable photostability than its tetramethyl or azatidine analogue, at the same time completely resistant to oxidative photobluing rendered by the Bredt's rule. Functionalized BD dyes are tailored for applications in single-molecule imaging, super-resolution imaging (STED and SIM) in fixed or live mammalian cells and plant cells, and live zebrafish imaging or chemigenetic voltage imaging. Synergizing with advanced imaging methods, the bridge bicycle dyes represent a versatile palette for biological researches. |
| 语种 | 英语 |
| DOI | 10.1101/2024.12.20.629585 |
| 相关网址 | 查看原文 |
| 出处 | bioRxiv |
| 收录类别 | PPRN.PPRN |
| WOS记录号 | PPRN:120090807 |
| WOS类目 | Biophysics |
| 资助项目 | National Key R&D Program of China["2021YFF0502904","2020YFA0509502"] ; Beijing Municipal Science & Technology Commission[Z221100003422013] ; National Natural Science Foundation of China[32170566] |
| 文献类型 | 预印本 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/483979 |
| 专题 | 上海科技大学 |
| 通讯作者 | Chen, Zhixing |
| 作者单位 | 1.Peking Univ, Inst Mol Med, Coll Future Technol, Natl Biomed Imaging Ctr,Beijing Key Lab Cardiometab Mol Med, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Neurosci, Ctr Excellence Brain Sci & Intelligence Technol, State Key Lab Neurosci, Shanghai, Peoples R China 3.Peking Univ, Acad Adv Interdisciplinary Studies, Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China 4.Peking Univ, Biomed Pioneering Innovat Ctr BIOP, Beijing, Peoples R China 5.Peking Univ, PKU IDG McGovern Inst Brain Res, Coll Chem & Mol Engn,Key Lab Bioorgan Chem & Mol Engn,Minist Educ, Synthet & Funct Biomol,Ctr Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 6.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing, Peoples R China 7.BSJ Inst, Beijing, Peoples R China 8.PKU Nanjing Inst Translat Med, Nanjing, Peoples R China 9.Genvivo Biotech, Nanjing, Peoples R China 10.ShanghaiTech Univ, Shanghai, Peoples R China 11.Chinese Inst Brain Res CIBR, Beijing 102206, Peoples R China 12.Peking Univ, Beijing Adv Innovat Ctr Genom ICG, Beijing, Peoples R China 13.Univ Chinese Acad Sci, Beijing, Peoples R China 14.Peking Univ, Coll Future Technol, Dept Biomed Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Junwei,Zhang, Kecheng,Wang, Kui,et al. A Palette of Bridged Bicycle-Strengthened Fluorophores. 2024. |
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