Rab2B promotes chaperonin-mediated actin folding and prevents developmental transcription reprogramming and quiescence in trypanosomes

doi:10.1101/2024.11.27.625582

	Rab2B promotes chaperonin-mediated actin folding and prevents developmental transcription reprogramming and quiescence in trypanosomes
	Li, Feng-Jun 1; Dong, Xiaoduo1,3 ; Huang, Yameng 1; Hu, Donghui 1; Chae, Eunyoung 1; He, Cynthia Y 1,2
	2024-11-28
状态	已发表
摘要	The Rab family of small GTPases are regulators of intracellular membrane trafficking. Rab2B, a Golgi-resident Rab GTPase, has been shown to function in ER-Golgi transport and autophagosome-lysosome fusion in mammalian cells and Drosophila. In Trypanosoma brucei, the autophagy-related function of Rab2B was conserved and depletion of Rab2B blocked autophagosome-lysosome fusion. Intriguingly, depletion of Rab2B induced differentiation of T. brucei from a proliferative slender form to a quiescent stumpy form, a step crucial for parasite transmission. Chaperonin CCT/TRiC was identified as an effector of Rab2B. Depletion of CCT/TRiC subunits or actin (a known CCT/TRiC substrate), or blocking actin polymerisation with Latrunculin A all triggered differentiation, suggesting a role of Rab2B on CCT/TRiC-mediated actin folding. Using an actin chromobody, we identified a pool of nuclear actin that is crucial for transcription reprogramming and life cycle differentiation in T. brucei. The study revealed a function of Rab2B and actin in T. brucei transcription reprogramming leading to cell differentiation, and an unexpected role of Rab2B and CCT/TRiC mediated actin folding.
关键词	Rab2B autophagy differentiation chaperonin (CCT/TRiC) actin folding RNA polymerase II transcription Trypanosoma brucei life cycle
语种	英语
DOI	10.1101/2024.11.27.625582
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:119464244
WOS类目	Cell Biology
资助项目	Ministry of Education, Singapore["MOE-T2EP30221-0002","A-0008403-00-00"]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/471037
专题	iHuman研究所_PI研究组_张潇洁组
通讯作者	He, Cynthia Y
作者单位	1.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore 2.Natl Univ Singapore, Ctr BioImaging Sci, Singapore 117543, Singapore 3.Shanghai Tech Univ, iHuman Inst, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Li, Feng-Jun,Dong, Xiaoduo,Huang, Yameng,et al. Rab2B promotes chaperonin-mediated actin folding and prevents developmental transcription reprogramming and quiescence in trypanosomes. 2024.