ALCAM is an entry factor for severe community acquired Pneumonia-associated Human adenovirus species B

doi:10.1038/s41467-024-55261-3

KMS > 免疫化学研究所

	ALCAM is an entry factor for severe community acquired Pneumonia-associated Human adenovirus species B
	Xie, Yusang 1,2; Mei, Hong3,4 ; Wang, Wei3,4 ; Li, Xiao 5; Hu, Pengfei3,4 ; Tian, Xingui 5; Zhou, Rong 5,6; Liu, Jia3,4,6,7 ; Qu, Jieming 1,2
	2024-12-30
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
EISSN	2041-1723
卷号	15 期号:1
DOI	10.1038/s41467-024-55261-3
摘要	Human adenovirus (HAdV) is a widely spread respiratory pathogen that can cause infections in multiple tissues and organs. Previous studies have established an association between HAdV species B (HAdV-B) infection and severe community-acquired pneumonia (SCAP). However, the connection between SCAP-associated HAdV-B infection and host factor expression profile in patients has not been systematically investigated. Here, we perform a CRISPR genetic screen on HAdV-B using two generations of cell surface protein-focused CRISPR libraries and identify a series of host factors including the known receptor DSG-2 and an unknown factor, activated leukocyte cell adhesion molecule (ALCAM). Further investigation shows that ALCAM affects HAdV-B infection by participating in viral internalization. Transcriptomics data from human blood samples suggests that ALCAM expression is higher in SCAP patients with HAdV-B infection than in those with other infections. Chimeric and authentic virus experiments show that ALCAM is a widely used host factor across B1 and B2 genetic clusters of HAdV-B. The dissociation constant between the knob domain of HAdV-B fiber and ALCAM is 837 nM in average. In summary, our results suggest that ALCAM is an entry factor for SCAP-associated HAdV-B.
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收录类别	SCI
语种	英语
资助项目	ShanghaiTech University[2023ZD0506200] ; National Key Research and Development Program of China[GZNL2024A01014] ; Major Project of Guangzhou National Laboratory[shslczdzk0220] ; Shanghai Municipal Key Clinical Specialty[2017ZZ02014] ; Shanghai Top-Priority Clinical Key Disciplines Construction Project[20dz2261100] ; Shanghai Key Laboratory of Emergency Prevention, Diagnosis and Treatment of Respiratory Infectious Diseases[20dz2210500] ; Innovative Research Team of High-level Local Universities in Shanghai[23ZR1442100] ; Science and Technology Commission of Shanghai Municipality[2022A0301-417-01]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001386373100037
出版者	NATURE PORTFOLIO
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/470981
专题	免疫化学研究所生命科学与技术学院生命科学与技术学院_博士生免疫化学研究所_公共科研平台_生物医学大数据平台免疫化学研究所_PI研究组_刘佳组
通讯作者	Liu, Jia; Qu, Jieming
作者单位	1.Shanghai Jiao Tong Univ, Ruijin Hosp, Inst Resp Dis, Sch Med,Dept Pulm & Crit Care Med, Shanghai, Peoples R China 2.Shanghai Key Lab Emergency Prevent, Diag & Treatment Resp Infect Dis, Shanghai, Peoples R China 3.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 5.Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Hlth, Natl Clin Res Ctr Resp Dis,State Key Lab Resp Dis, Guangzhou, Peoples R China 6.Guangzhou Int Bio Isl, Guangzhou Natl Lab, 9 XingDaoHuanBei Rd, Guangzhou, Guangdong, Peoples R China 7.Shanghai Clin Res & Trial Ctr, Shanghai, Peoples R China
通讯作者单位	免疫化学研究所; 生命科学与技术学院
推荐引用方式 GB/T 7714	Xie, Yusang,Mei, Hong,Wang, Wei,et al. ALCAM is an entry factor for severe community acquired Pneumonia-associated Human adenovirus species B[J]. NATURE COMMUNICATIONS,2024,15(1).
APA	Xie, Yusang.,Mei, Hong.,Wang, Wei.,Li, Xiao.,Hu, Pengfei.,...&Qu, Jieming.(2024).ALCAM is an entry factor for severe community acquired Pneumonia-associated Human adenovirus species B.NATURE COMMUNICATIONS,15(1).
MLA	Xie, Yusang,et al."ALCAM is an entry factor for severe community acquired Pneumonia-associated Human adenovirus species B".NATURE COMMUNICATIONS 15.1(2024).