Trimethylsilyl reporter groups for NMR studies of conformational changes in G protein-coupled receptors

doi:10.1002/1873-3468.13382

KMS > iHuman研究所 > 特聘教授组 > Kurt Wuthrich组

	Trimethylsilyl reporter groups for NMR studies of conformational changes in G protein-coupled receptors
	Hu, Wanhui1 ; Wang, Huixia1,2,3 ; Hou, Yaguang1,2,3 ; Hao, Yimei1,2,3 ; Liu, Dongsheng1
	2019-05
发表期刊	FEBS LETTERS (IF:3.0[JCR-2023],3.8[5-Year])
ISSN	0014-5793
卷号	593 期号:10 页码:1113-1121
发表状态	已发表
DOI	10.1002/1873-3468.13382
摘要	Large membrane proteins such as G protein-coupled receptors (GPCRs) are difficult for NMR study due to severe signal overlaps and unfavorable relaxation properties. We used a trimethylsilyl (TMS) group as a reporter group for H-1 NMR study of conformational changes in proteins, utilizing high-intensity H-1 NMR signals near 0 p.p.m. The beta(2)-adrenergic receptor was labeled with TMS groups at two cysteines located at the cytoplasmic ends of helices VI and VII. Binding of various ligands led to changes in H-1 NMR signals, which manifested that helix VI is sensitive to G protein-specific activation, whereas helix VII is sensitive to beta-arrestin-specific activation. Thus, the TMS group is a useful reporter group in NMR for studying conformational changes in membrane proteins such as GPCRs.
关键词	G protein-coupled receptor nuclear magnetic resonance trimethylsilylmethanethiol beta(2)-adrenergic receptor
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31670733]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号	WOS:000469263700011
出版者	WILEY
WOS关键词	BETA(2)-ADRENERGIC RECEPTOR ; CRYSTAL-STRUCTURE ; DYNAMIC PROCESS ; F-19 NMR ; BETA(2) ; AGONISTS ; BINDING ; MECHANISMS ; INSIGHTS ; COMPLEX
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/45620
专题	iHuman研究所_特聘教授组_Kurt Wuthrich组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Liu, Dongsheng
作者单位	1.ShanghaiTech Univ, iHuman Inst, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	iHuman研究所
通讯作者单位	iHuman研究所
第一作者的第一单位	iHuman研究所
推荐引用方式 GB/T 7714	Hu, Wanhui,Wang, Huixia,Hou, Yaguang,et al. Trimethylsilyl reporter groups for NMR studies of conformational changes in G protein-coupled receptors[J]. FEBS LETTERS,2019,593(10):1113-1121.
APA	Hu, Wanhui,Wang, Huixia,Hou, Yaguang,Hao, Yimei,&Liu, Dongsheng.(2019).Trimethylsilyl reporter groups for NMR studies of conformational changes in G protein-coupled receptors.FEBS LETTERS,593(10),1113-1121.
MLA	Hu, Wanhui,et al."Trimethylsilyl reporter groups for NMR studies of conformational changes in G protein-coupled receptors".FEBS LETTERS 593.10(2019):1113-1121.