Cell cycle- and genomic distance-dependent dynamics of a discrete chromosomal region

doi:10.1083/jcb.201807162

	Cell cycle- and genomic distance-dependent dynamics of a discrete chromosomal region
	Ma, Hanhui1,5 ; Tu, Li-Chun 2,6; Chung, Yu-Chieh 4,6; Naseri, Ardalan 3; Grunwald, David 2; Zhang, Shaojie 3; Pederson, Thoru 1
	2019-05
发表期刊	JOURNAL OF CELL BIOLOGY
ISSN	0021-9525
卷号	218 期号:5 页码:1467-1477
发表状态	已发表
DOI	10.1083/jcb.201807162
摘要	In contrast to the well-studied condensation and folding of chromosomes during mitosis, their dynamics during interphase are less understood. We deployed a CRISPR-based DNA imaging system to track the dynamics of genomic loci situated kilobases to megabases apart on a single chromosome. Two distinct modes of dynamics were resolved: local movements as well as ones that might reflect translational movements of the entire domain within the nucleoplasmic space. The magnitude of both of these modes of movements increased from early to late G1, whereas the translational movements were reduced in early S phase. The local fluctuations decreased slightly in early S and more markedly in mid-late S. These newly observed movements and their cell cycle dependence suggest the existence of a hitherto unrecognized compaction-relaxation dynamic of the interphase chromosome fiber, operating concurrently with changes in the extent of overall movements of loci in the 4D genome.
收录类别	SCI ; SCIE
语种	英语
资助项目	National Institutes of Health[K99 GM126810] ; National Institutes of Health[U01 DA-040588] ; National Institutes of Health[R01 GM102515] ; National Institutes of Health[U01 EB021238]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000466980400008
出版者	ROCKEFELLER UNIV PRESS
WOS关键词	CHROMATIN DECOMPACTION ; IMAGING REVEALS ; ORGANIZATION ; VISUALIZATION ; TERRITORIES ; PRINCIPLES ; BINDING
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/45616
专题	生命科学与技术学院_PI研究组_马涵慧组
通讯作者	Pederson, Thoru
作者单位	1.Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA 01655 USA 2.Univ Massachusetts, RNA Therapeut Inst, Med Sch, Worcester, MA USA 3.Univ Cent Florida, Dept Comp Sci, Orlando, FL 32816 USA 4.Univ Tokyo, Kavli Inst Phys & Math Universe, Kashiwa, Chiba, Japan 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 6.Ohio State Univ, Dept Biol Chem & Pharmacol, Columbus, OH 43210 USA
第一作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Ma, Hanhui,Tu, Li-Chun,Chung, Yu-Chieh,et al. Cell cycle- and genomic distance-dependent dynamics of a discrete chromosomal region[J]. JOURNAL OF CELL BIOLOGY,2019,218(5):1467-1477.
APA	Ma, Hanhui.,Tu, Li-Chun.,Chung, Yu-Chieh.,Naseri, Ardalan.,Grunwald, David.,...&Pederson, Thoru.(2019).Cell cycle- and genomic distance-dependent dynamics of a discrete chromosomal region.JOURNAL OF CELL BIOLOGY,218(5),1467-1477.
MLA	Ma, Hanhui,et al."Cell cycle- and genomic distance-dependent dynamics of a discrete chromosomal region".JOURNAL OF CELL BIOLOGY 218.5(2019):1467-1477.