Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling
Gao, Hong1; Yin, Fen-Fen1,2; Guan, Dong-Xian1; Feng, Yu-Xiong1; Zheng, Qian-Wen3; Wang, Xiang4; Zhu, Min4; Zhang, Xue-Li5; Cheng, Shu-Qun6; Chen, Tian-Wei1,2; Jiang, Hao1; Zhang, Er-Bin1,2; Wang, Jing-Jing1; Ni, Qian-Zhi1,2; Yuan, Yan-Mei1,2; Zhang, Feng-Kun1,2; Ma, Ning1,2; Cao, Hui-Jun1,2; Wang, Yi-Kang1,2; Li, Jing-Jing1; Xie, Dong1,2,3,7
2019-05
Source PublicationCELL PROLIFERATION
ISSN0960-7722
Volume52Issue:3
Status已发表
DOI10.1111/cpr.12583
AbstractObjectives Wnt1-inducible signalling pathway protein 3 (WISP3/CCN6) belongs to the CCN (CYR61/CTGF/NOV) family of proteins, dysregulation of this family contributed to the tumorigenicity of various tumours. In this study, we need to explore its role in hepatocellular carcinoma that remains largely elusive. Materials and Methods The expression of WISP3/CCN6 was analysed by qRT-PCR and Western blotting. Effects of WISP3 on proliferation and metastasis of HCC cells were examined, respectively, by MTT assay and Boyden Chamber. Roles of WISP3 on HCC tumour growth and metastatic ability in vivo were detected in nude mice. Related mechanism study was confirmed by immunofluorescence and Western blotting. Results The expression of WISP3 was significantly downregulated in HCC clinical samples and cell lines, and reversely correlated with the tumour size. Forced expression of WISP3 in HCC cells significantly suppressed cell growth and migration in vitro as well as tumour growth and metastatic seeding in vivo. In contrast, downregulation of WISP3 accelerated cell proliferation and migration, and promoted in vivo metastasis. Further study revealed that WISP3 inhibited the translocation of beta-catenin to the nucleus by activating glycogen synthase kinase-3 beta (GSK3 beta). Moreover, constitutively active beta-catenin blocked the suppressive effects of WISP3 on HCC. Conclusions Our study showed that WISP3 suppressed the progression of HCC by negative regulation of beta-catenin/TCF/LEF signalling, providing WISP3 as a potential therapeutic candidate for HCC.
Keywordhepatocellular carcinoma metastasis tumorigenesis WISP3 beta-catenin
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31520103907] ; National Natural Science Foundation of China[81730083] ; National Natural Science Foundation of China[31771538] ; National Natural Science Foundation of China[31601143]
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000469271700015
PublisherWILEY
WOS KeywordCCN FAMILY ; E-CADHERIN ; COLORECTAL CARCINOMAS ; WNT PATHWAY ; CATENIN ; PROLIFERATION ; EXPRESSION ; GROWTH ; CELLS ; GENE
Original Document TypeArticle
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://kms.shanghaitech.edu.cn/handle/2MSLDSTB/45615
Collection生命科学与技术学院_硕士生
生命科学与技术学院_特聘教授组_谢东组
Corresponding AuthorLi, Jing-Jing; Xie, Dong
Affiliation1.Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Shanghai Inst Biol Sci, CAS Key Lab Nutr Metab & Food Safety, Shanghai, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai, Peoples R China
3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
4.Huzhou Univ, Peoples Hosp 1, Dept Surg, Huzhou, Peoples R China
5.Southern Med Univ, Fengxian Hosp, Dept Gen Surg, Shanghai, Peoples R China
6.Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Shanghai, Peoples R China
7.China Natl Ctr Food Safety Risk Assessment, NHC Key Lab Food Safety Risk Assessment, Beijing, Peoples R China
Corresponding Author AffilicationSchool of Life Science and Technology
Recommended Citation
GB/T 7714
Gao, Hong,Yin, Fen-Fen,Guan, Dong-Xian,et al. Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling[J]. CELL PROLIFERATION,2019,52(3).
APA Gao, Hong.,Yin, Fen-Fen.,Guan, Dong-Xian.,Feng, Yu-Xiong.,Zheng, Qian-Wen.,...&Xie, Dong.(2019).Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling.CELL PROLIFERATION,52(3).
MLA Gao, Hong,et al."Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling".CELL PROLIFERATION 52.3(2019).
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