Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling

doi:10.1111/cpr.12583

	Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling
	Gao, Hong 1; Yin, Fen-Fen 1,2; Guan, Dong-Xian 1; Feng, Yu-Xiong 1; Zheng, Qian-Wen3 ; Wang, Xiang 4; Zhu, Min 4; Zhang, Xue-Li 5; Cheng, Shu-Qun 6; Chen, Tian-Wei 1,2; Jiang, Hao 1; Zhang, Er-Bin 1,2; Wang, Jing-Jing 1; Ni, Qian-Zhi 1,2; Yuan, Yan-Mei 1,2; Zhang, Feng-Kun 1,2; Ma, Ning 1,2; Cao, Hui-Jun 1,2; Wang, Yi-Kang 1,2; Li, Jing-Jing 1; Xie, Dong1,2,3,7
	2019-05
发表期刊	CELL PROLIFERATION
ISSN	0960-7722
卷号	52 期号:3
发表状态	已发表
DOI	10.1111/cpr.12583
摘要	Objectives Wnt1-inducible signalling pathway protein 3 (WISP3/CCN6) belongs to the CCN (CYR61/CTGF/NOV) family of proteins, dysregulation of this family contributed to the tumorigenicity of various tumours. In this study, we need to explore its role in hepatocellular carcinoma that remains largely elusive. Materials and Methods The expression of WISP3/CCN6 was analysed by qRT-PCR and Western blotting. Effects of WISP3 on proliferation and metastasis of HCC cells were examined, respectively, by MTT assay and Boyden Chamber. Roles of WISP3 on HCC tumour growth and metastatic ability in vivo were detected in nude mice. Related mechanism study was confirmed by immunofluorescence and Western blotting. Results The expression of WISP3 was significantly downregulated in HCC clinical samples and cell lines, and reversely correlated with the tumour size. Forced expression of WISP3 in HCC cells significantly suppressed cell growth and migration in vitro as well as tumour growth and metastatic seeding in vivo. In contrast, downregulation of WISP3 accelerated cell proliferation and migration, and promoted in vivo metastasis. Further study revealed that WISP3 inhibited the translocation of beta-catenin to the nucleus by activating glycogen synthase kinase-3 beta (GSK3 beta). Moreover, constitutively active beta-catenin blocked the suppressive effects of WISP3 on HCC. Conclusions Our study showed that WISP3 suppressed the progression of HCC by negative regulation of beta-catenin/TCF/LEF signalling, providing WISP3 as a potential therapeutic candidate for HCC.
关键词	hepatocellular carcinoma metastasis tumorigenesis WISP3 beta-catenin
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31520103907] ; National Natural Science Foundation of China[81730083] ; National Natural Science Foundation of China[31771538] ; National Natural Science Foundation of China[31601143]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000469271700015
出版者	WILEY
WOS关键词	CCN FAMILY ; E-CADHERIN ; COLORECTAL CARCINOMAS ; WNT PATHWAY ; CATENIN ; PROLIFERATION ; EXPRESSION ; GROWTH ; CELLS ; GENE
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/45615
专题	生命科学与技术学院_特聘教授组_谢东组生命科学与技术学院_硕士生
通讯作者	Li, Jing-Jing; Xie, Dong
作者单位	1.Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Shanghai Inst Biol Sci, CAS Key Lab Nutr Metab & Food Safety, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Huzhou Univ, Peoples Hosp 1, Dept Surg, Huzhou, Peoples R China 5.Southern Med Univ, Fengxian Hosp, Dept Gen Surg, Shanghai, Peoples R China 6.Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Shanghai, Peoples R China 7.China Natl Ctr Food Safety Risk Assessment, NHC Key Lab Food Safety Risk Assessment, Beijing, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Gao, Hong,Yin, Fen-Fen,Guan, Dong-Xian,et al. Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling[J]. CELL PROLIFERATION,2019,52(3).
APA	Gao, Hong.,Yin, Fen-Fen.,Guan, Dong-Xian.,Feng, Yu-Xiong.,Zheng, Qian-Wen.,...&Xie, Dong.(2019).Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling.CELL PROLIFERATION,52(3).
MLA	Gao, Hong,et al."Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling".CELL PROLIFERATION 52.3(2019).