上海科技大学知识管理系统

本发明属于生物制药领域，公开了C 2 C 9核酸酶作为RNA引导的核酸内切酶的用途以及一种极小基因组编辑系统，所述基因组编辑系统包括编码C 2 C 9核酸酶的核酸和//或C 2 C 9核酸酶，以及引导RNA或编码引导RNA的核酸。本发明所述的超小型基因组编辑系统能够针对原核细菌和真核细胞基因组中的至少一个靶序列进行基因编辑。本发明还公开了该基因组编辑系统的用途和编辑方法。通过本发明的基因编辑系统或方法可以精确地敲除或切割目的基因，且切割效率高，实现了目的基因的精确编辑[选择图]无

The present invention belongs to the field of biopharmaceuticals and discloses the use of C 2 C 9 nuclease used as an RNA guided endonuclease and a very small genome editing system, wherein the genome editing system includes a nucleic acid encoding a C 2 C 9 nuclease and // or the C 2 C 9 nuclease, and a guide RNA or a nucleic acid encoding the guide RNA. The ultra-small genome editing system described in the present invention can perform gene editing for at least one target sequence in the genome of prokaryotic bacteria and eukaryotic cells. The present invention also discloses the use and editing method of the genome editing system. The target gene can be accurately knocked out or cleaved by the gene editing system or method of the present invention, and the cutting efficiency is high and accurate editing of the target gene is realized.[Selection Diagram]None