Exploration of glyoxals’ level changes in endoplasmic reticulum during ferroptosis by a photocaged fluorescent probe

doi:10.1016/j.snb.2024.136745

	Exploration of glyoxals’ level changes in endoplasmic reticulum during ferroptosis by a photocaged fluorescent probe
	Xing, Wanjin 1; Zhang, Yirong 1; Ma, Huijuan 1; Shen, Shuran 1; Wang, Sinan2 ; Xu, Huan 3; Wang, Wei 4; Lou, Kaiyan 1
	2025-01-15
发表期刊	SENSORS AND ACTUATORS B: CHEMICAL (IF:8.0[JCR-2023],7.0[5-Year])
ISSN	0925-4005
EISSN	0925-4005
卷号	423
发表状态	已发表
DOI	10.1016/j.snb.2024.136745
摘要	Both ferroptosis and elevated glyoxals (e.g. methylglyoxal and glyoxal), are associated with endoplasmic reticulum stress, but their correlations, in particular, how level of glyoxals changes in endoplasmic reticulum during ferroptosis remains unclear. In this work, a new photoactivatable fluorescent probe Photo-ER-GOS was developed to study level changes of glyoxals in endoplasmic reticulum during ferroptosis via in situ photodecaging and fluorescence detection, avoiding potential interference from glyoxals in cytosol. The probe contains a photolabile nitrobenzyloxymethyl group functioned both as the reactivity blocking group and the fluorescence quenching group, which linked to the guanidino group of NAP-DCP-3, the active endoplasmic reticulum-targeting fluorescent probe for glyoxals previously developed in our lab. It was found that the level of glyoxals in endoplasmic reticulum increases significantly during erastin-induced ferroptosis in RAW 264.7 cells via comparison of relative fluorescence intensity increases. The endoplasmic reticulum glyoxal level increase was also confirmed by the Ultra Performance Liquid Chromatography-Mass Spectrum studies. In contrast, no significant change of level of glyoxals in endoplasmic reticulum was found in staurosporine-induced apoptosis, suggesting that upregulated glyoxals in endoplasmic reticulum may be a previously overlooked characteristic of ferroptosis. Erastin-induced level increase of glyoxals was also found in zebrafish. The caged probe Photo-ER-GOS thus provides a useful chemical tool to study GOS levels changes in endoplasmic reticulum. © 2024 Elsevier B.V.
关键词	Cell death Chemical detection Dicarbonyl stress Dicarbonyls Endoplasmic reticulum Endoplasmic reticulum stress Ferroptosis Fluorescent probes Glyoxal Level change Methylglyoxal Photocaged fluorescent probe
URL	查看原文
收录类别	EI ; SCI
语种	英语
资助项目	Shanghai Natural Science Fund[20ZR1414700] ; National Natural Science Foundation of China[42177417] ; Undergraduate Innovation and Entrepreneurship Training Program, ECUST[X202310251130]
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:001335317500001
出版者	Elsevier B.V.
EI入藏号	20244117178662
EI主题词	Liquid chromatography
EI分类号	103 ; 802 Chemical Apparatus and Plants ; Unit Operations ; Unit Processes ; 802.3 Chemical Operations
原始文献类型	Journal article (JA)
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/436532
专题	生物医学工程学院_PI研究组_王斯楠组
通讯作者	Xu, Huan
作者单位	1.State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, Shanghai Key Laboratory of New Drug Design, and Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science & Technology, 130 Meilong Road, Shanghai; 200237, China; 2.School of Biomedical Engineering & State Key Laboratory of Advanced Medical Materials and Devices, ShanghaiTech University, 393 Huaxia Road, Shanghai; 201210, China; 3.School of Public Health, Anhui University of Science and Technology, Anhui Province, Hefei; 231131, China; 4.Department of Pharmacology and Toxicology and BIO5 Institute, University of Arizona, Tucson; AZ; 85721-0207, United States
推荐引用方式 GB/T 7714	Xing, Wanjin,Zhang, Yirong,Ma, Huijuan,et al. Exploration of glyoxals’ level changes in endoplasmic reticulum during ferroptosis by a photocaged fluorescent probe[J]. SENSORS AND ACTUATORS B: CHEMICAL,2025,423.
APA	Xing, Wanjin.,Zhang, Yirong.,Ma, Huijuan.,Shen, Shuran.,Wang, Sinan.,...&Lou, Kaiyan.(2025).Exploration of glyoxals’ level changes in endoplasmic reticulum during ferroptosis by a photocaged fluorescent probe.SENSORS AND ACTUATORS B: CHEMICAL,423.
MLA	Xing, Wanjin,et al."Exploration of glyoxals’ level changes in endoplasmic reticulum during ferroptosis by a photocaged fluorescent probe".SENSORS AND ACTUATORS B: CHEMICAL 423(2025).