Dynamic entity formed by protein and its hydration water

doi:10.1103/PhysRevResearch.6.033316

	Dynamic entity formed by protein and its hydration water
	Ye, Yongfeng 1,2; Chen, Xiaoxia 2; Huang, Juan 3; Zheng, Lirong 1,2; Tang, Qingxue 4; Long, Liuliu5 ; Yamada, Takeshi 6; Tyagi, Madhusudan 7,8; Sakai, Victoria García 9; O'Neill, Hugh 10; Zhang, Qiu 10; De Souza, Nicolas R.11; Xiao, Xiang 4,12,13; Zhao, Weishu 4,12,13; Hong, Liang 1,2,14,15; Liu, Zhuo 2,14,15
	2024-07
发表期刊	PHYSICAL REVIEW RESEARCH (IF:3.5[JCR-2023],3.8[5-Year])
ISSN	2643-1564
卷号	6 期号:3
发表状态	已发表
DOI	10.1103/PhysRevResearch.6.033316
摘要	The interaction between protein and water plays a pivotal role in shaping the structure, dynamics, and function of biomacromolecules. A comprehensive understanding of this intricate interplay necessitates a systematic evaluation of interaction strength and its consequential impact on the dynamics of proteins and water across diverse protein systems. Despite numerous works on understanding the dynamics of water and proteins and the coupling between them, there are still unanswered questions. Here, we combine neutron scattering and isotope labeling to probe the dynamics of proteins and their hydration water in a variety of protein systems. We consider proteins of different structures and varying thermostability as well as proteins within living cells with distinct growth temperatures. Simultaneous characterization of protein and hydration water dynamics across diverse systems was achieved. Moreover, we performed water sorption isothermal measurements on three representative proteins to correlate the observed dynamics with the strength of the interaction energies governing each system. The experimental results underscore that proteins manifesting stronger attractive interactions with water display diffusionlike dynamics with higher flexibility upon hydration, concomitant with a reduced mobility in hydration water. Significantly, our findings suggest that, in fact, it is the interaction between protein and its hydration water that facilitates the transfer of mobility from water to protein, with stronger interactions correlating to greater protein flexibility and slower hydration water diffusion. © 2024 authors.
关键词	Neutron scattering Biomacromolecule Different structure Diverse proteins Hydration water Interaction strength Isotope labeling Protein system Structure dynamics Structure functions Systematic evaluation
收录类别	EI
语种	英语
出版者	American Physical Society
EI入藏号	20243917095293
EI主题词	Hydration
EI分类号	801.3 Colloid Chemistry ; 802.2 Chemical Reactions
原始文献类型	Journal article (JA)
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/430488
专题	物质科学与技术学院物质科学与技术学院_公共科研平台_分析测试平台
通讯作者	Zhao, Weishu; Hong, Liang; Liu, Zhuo
作者单位	1.School of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai; 200240, China 2.Institute of Natural Sciences, Shanghai Jiao Tong University, Shanghai; 200240, China 3.School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai; 200240, China 4.State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai; 200240, China 5.School of Physical Science and Technology, ShanghaiTech University, Shanghai; 201210, China 6.Neutron Science and Technology Center, Comprehensive Research Organization for Science and Society, 162-1 Shirakata, Tokai, Naka, Ibaraki; 319-1106, Japan 7.National Institute of Standards and Technology (NIST), NIST Center for Neutron Research, Gaithersburg; MD; 20899, United States 8.Department of Materials Science and Engineering, University of Maryland, College Park; MD; 20742, United States 9.ISIS Pulsed Neutron and Muon Source, Rutherford Appleton Laboratory, Science and Technology Facilities Council, Didcot; OX11 0QX, United Kingdom 10.Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge; TN; 37931, United States 11.Australian Nuclear Science and Technology (ANSTO), Locked Bag 2001, DC, Kirrawee; NSW; 2232, Australia 12.International Center for Deep Life Investigation, Shanghai Jiao Tong University, Shanghai; 200240, China 13.SJTU Yazhou Bay Institute of Deepsea Sci-Tech, Yongyou Industrial Park, Sanya; 572024, China 14.Shanghai National Centre for Applied Mathematics (SJTU Center), MOE-LSC, Shanghai Jiao Tong University, Shanghai; 200240, China 15.Zhangjiang Institute for Advanced Study, Shanghai Jiao Tong University, Shanghai; 201203, China
推荐引用方式 GB/T 7714	Ye, Yongfeng,Chen, Xiaoxia,Huang, Juan,et al. Dynamic entity formed by protein and its hydration water[J]. PHYSICAL REVIEW RESEARCH,2024,6(3).
APA	Ye, Yongfeng.,Chen, Xiaoxia.,Huang, Juan.,Zheng, Lirong.,Tang, Qingxue.,...&Liu, Zhuo.(2024).Dynamic entity formed by protein and its hydration water.PHYSICAL REVIEW RESEARCH,6(3).
MLA	Ye, Yongfeng,et al."Dynamic entity formed by protein and its hydration water".PHYSICAL REVIEW RESEARCH 6.3(2024).