Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei

doi:10.1016/j.stem.2024.08.004

	Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei
	Wei Pang; Jinkui Zhu; Kexin Yang; Xiaona Zhu; Wei Zhou; Linlin Jiang; Xuran Zhuang; Yantong Liu; Jianfeng Wei; Xiaoxiang Lu; Yao Yin; Ziling Chen; Yangfei Xiang
	2024-10-03
发表期刊	CELL STEM CELL (IF:19.8[JCR-2023],22.6[5-Year])
ISSN	1934-5909
EISSN	1875-9777
卷号	31 期号:10
发表状态	已发表
DOI	10.1016/j.stem.2024.08.004
摘要	Brain organoids with nucleus-specific identities provide unique platforms for studying human brain development and diseases at a finer resolution. Despite its essential role in vital body functions, the medulla of the hindbrain has seen a lack of in vitro models, let alone models resembling specific medullary nuclei, including the crucial spinal trigeminal nucleus (SpV) that relays peripheral sensory signals to the thalamus. Here, we report a method to differentiate human pluripotent stem cells into region-specific brain organoids resembling the dorsal domain of the medullary hindbrain. Importantly, organoids specifically recapitulated the development of the SpV derived from the dorsal medulla. We also developed an organoid system to create the trigeminothalamic projections between the SpV and the thalamus by fusing these organoids, namely human medullary SpV-like organoids (hmSpVOs), with organoids representing the thalamus (hThOs). Our study provides a platform for understanding SpV development, nucleus-based circuit organization, and related disorders in the human brain.
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收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China, China[2021YFF1200800] ; National Natural Science Foundation of China, China[32170836] ; Central Guidance on Local Science and Technology Development Fund, China[YDZX20233100001002] ; Shanghai Pujiang Program, China[20PJ1410400]
WOS研究方向	Cell Biology
WOS类目	Cell & Tissue Engineering ; Cell Biology
WOS记录号	WOS:001331485900001
出版者	CELL PRESS
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/421380
专题	生命科学与技术学院_博士生生命科学与技术学院_PI研究组_胡霁组生命科学与技术学院_硕士生生命科学与技术学院_PI研究组_向阳飞组
共同第一作者	Jinkui Zhu
通讯作者	Yangfei Xiang
作者单位	1.School of Life Science and Technology, ShanghaiTech University 2.State Key Laboratory of Advanced Medical Materials and Devices, ShanghaiTech University 3.Shanghai Clinical Research and Trial Center
推荐引用方式 GB/T 7714	Wei Pang,Jinkui Zhu,Kexin Yang,et al. Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei[J]. CELL STEM CELL,2024,31(10).
APA	Wei Pang.,Jinkui Zhu.,Kexin Yang.,Xiaona Zhu.,Wei Zhou.,...&Yangfei Xiang.(2024).Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei.CELL STEM CELL,31(10).
MLA	Wei Pang,et al."Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei".CELL STEM CELL 31.10(2024).