上海科技大学知识管理系统

一种基于微型CRISPR Cas蛋白IscB的高效碱基编辑系统，该IscBn-ωRNA编辑系统的IscBn(D61A)存在D96K//Q99R//S456R//D89R//N462H//S430K//S386R//N384R//F137N这些点突变中的一种或多种，和//或其系统的ωRNA存在突变中的一种或多种，该IscBn(D61A)其氨基酸序列如SEQ ID No.1所示。该编辑系统在人细胞中实现高效的ABE、CBE、CGBE和AYBE的编辑能力。

An efficient base editing system on the basis of a miniature CRISPR Cas protein IscB, wherein IscBn (D61A) of the IscBn-ωRNA editing system has one or more of point mutations D96K//Q99R//S456R//D89R//N462H//S430K//S386R//N384R//F137N, and//or ωRNA of the system thereof has one or more of the mutations, and the amino acid sequence of the IscBn (D61A) is as shown in SEQ ID No.1. The editing system has efficient editing capabilities of ABE, CBE, CGBE and AYBE in human cells.