上海科技大学知识管理系统

本公开提供了用于制备和使用个体化的患者来源的肿瘤类器官(IPTO)的组合物和方法。人iPSC来源的脑类器官可用作肿瘤组织移植物的宿主。术后可直接获取患者材料，并解剖成小块。将所述小肿瘤组织插入所述脑类器官中，以制备杂合类器官。这种杂合类器官可以生长几周到几个月，即使是生长缓慢的肿瘤，如IDH突变的胶质瘤和毛细胞星形细胞瘤也能增殖。肿瘤块优选包括肿瘤细胞和邻近的基质细胞，从而允许测试常规化疗剂以及免疫治疗剂。

The present disclosure provides compositions and methods for preparing and using individualized patient-derived tumor organoids (IPTO). Human iPSC-derived cerebral organoids can be used as hosts for tumor tissue grafts. Patient materials can be obtained directly after surgery and dissected into small pieces. The small tumor tissue is inserted into the cerebral organoid to prepare a hybrid organoid. This hybrid organoid can grow for several weeks to months, allowing even slow-growing tumors, such as glioma with IDH mutations and pilocytic astrocytoma, to proliferate. The tumor pieces preferably include both tumor cells and adjacent stromal cells, allowing testing of conventional chemotherapeutic agents as well as immunotherapeutic agents.