Single-neuron projectome-guided analysis reveals the neural circuit mechanism underlying endogenous opioid antinociception

doi:10.1093/nsr/nwae195

	Single-neuron projectome-guided analysis reveals the neural circuit mechanism underlying endogenous opioid antinociception
	Dou, Yan-Nong 1; Liu, Yuan1,2,3 ; Ding, Wen-Qun 1,4; Li, Qing 1; Zhou, Hua 1; Li, Ling 1,4; Zhao, Meng-Ting 5; Li, Zheng-Yi-Qi 6; Yuan, Jing 5,7; Wang, Xiao-Fei 1; Zou, Wang-Yuan 6; Li, Anan 5,7; Sun, Yan-Gang 1
	2024-07-01
发表期刊	NATIONAL SCIENCE REVIEW (IF:16.3[JCR-2023],18.6[5-Year])
ISSN	2095-5138
EISSN	2053-714X
卷号	11 期号:7
发表状态	已发表
DOI	10.1093/nsr/nwae195
摘要	Endogenous opioid antinociception is a self-regulatory mechanism that reduces chronic pain, but its underlying circuit mechanism remains largely unknown. Here, we showed that endogenous opioid antinociception required the activation of mu-opioid receptors (MORs) in GABAergic neurons of the central amygdala nucleus (CEA) in a persistent-hyperalgesia mouse model. Pharmacogenetic suppression of these CEAMOR neurons, which mimics the effect of MOR activation, alleviated the persistent hyperalgesia. Furthermore, single-neuron projection analysis revealed multiple projectome-based subtypes of CEAMOR neurons, each innervating distinct target brain regions. We found that the suppression of axon branches projecting to the parabrachial nucleus (PB) of one subtype of CEAMOR neurons alleviated persistent hyperalgesia, indicating a subtype- and axonal-branch-specific mechanism of action. Further electrophysiological analysis revealed that suppression of a distinct CEA-PB disinhibitory circuit controlled endogenous opioid antinociception. Thus, this study identified the central neural circuit that underlies endogenous opioid antinociception, providing new insight into the endogenous pain modulatory mechanisms. © The Author(s) 2024.
关键词	Brain Chemical activation Electric network analysis Electrophysiology Timing circuits Antinociception Central amygdala nucleus Endogenous opioid antinociception Hyperalgesia Mu-opioid receptor Opioid receptors Opioids Parabrachial nucleus Single neuron Single-neuron projectome
URL	查看原文
收录类别	EI ; SCI
语种	英语
资助项目	National Science and Technology Innovation 2030 Major Program[2021ZD0204404] ; National Natural Science Foundation of China["82271244","32221003"] ; Shanghai Municipal Science and Technology Major Project[2018SHZDZX05] ; YangFan project[21YF1453200] ; Youth Innovation Promotion Association, Chinese Academy of Sciences[2022266]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001274234000001
出版者	Oxford University Press
EI入藏号	20243116781354
EI主题词	Neurons
EI分类号	461.1 Biomedical Engineering ; 461.9 Biology ; 703.1.1 Electric Network Analysis ; 713.4 Pulse Circuits ; 802.2 Chemical Reactions ; 804 Chemical Products Generally
原始文献类型	Journal article (JA)
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/407236
专题	生命科学与技术学院生命科学与技术学院_博士生
通讯作者	Dou, Yan-Nong
作者单位	1.Institute of Neuroscience, Key Laboratory of Brain Cognition and Brain-Inspired Intelligence Technology, CAS Center for Excellence in Brain Science & Intelligence Technology, Chinese Academy of Sciences, Shanghai; 200031, China; 2.Department of Biology, School of Life Science and Technology, ShanghaiTech University, Shanghai; 201210, China; 3.Lingang Laboratory, Shanghai; 200031, China; 4.University of Chinese Academy of Sciences, Beijing; 100049, China; 5.Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan; 430074, China; 6.Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha; 410008, China; 7.HUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou; 215123, China
推荐引用方式 GB/T 7714	Dou, Yan-Nong,Liu, Yuan,Ding, Wen-Qun,et al. Single-neuron projectome-guided analysis reveals the neural circuit mechanism underlying endogenous opioid antinociception[J]. NATIONAL SCIENCE REVIEW,2024,11(7).
APA	Dou, Yan-Nong.,Liu, Yuan.,Ding, Wen-Qun.,Li, Qing.,Zhou, Hua.,...&Sun, Yan-Gang.(2024).Single-neuron projectome-guided analysis reveals the neural circuit mechanism underlying endogenous opioid antinociception.NATIONAL SCIENCE REVIEW,11(7).
MLA	Dou, Yan-Nong,et al."Single-neuron projectome-guided analysis reveals the neural circuit mechanism underlying endogenous opioid antinociception".NATIONAL SCIENCE REVIEW 11.7(2024).