CoHIT: a one-pot ultrasensitive ERA-CRISPR system for detecting multiple same-site indels

doi:10.1038/s41467-024-49414-7

	CoHIT: a one-pot ultrasensitive ERA-CRISPR system for detecting multiple same-site indels
	Liu, Yin 1,2,3; Liu, Xinyi 4; Wei, Dongyi 5; Dang, Lu 6; Xu, Xiaoran 5; Huang, Shisheng 7; Li, Liwen 4; Wu, Sanyun 1; Wu, Jinxian 1; Liu, Xiaoyan 1; Sun, Wenjun8 ; Tao, Wanyu8 ; Wei, Yongchang 5; Huang, Xingxu8 ; Li, Kui 4; Wang, Xinjie 4; Zhou, Fuling 1
	2024-06-12
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
EISSN	2041-1723
卷号	15 期号:1
发表状态	已发表
DOI	10.1038/s41467-024-49414-7
摘要	["Genetic testing is crucial for precision cancer medicine. However, detecting multiple same-site insertions or deletions (indels) is challenging. Here, we introduce CoHIT (Cas12a-based One-for-all High-speed Isothermal Test), a one-pot CRISPR-based assay for indel detection. Leveraging an engineered AsCas12a protein variant with high mismatch tolerance and broad PAM scope, CoHIT can use a single crRNA to detect multiple NPM1 gene c.863_864 4-bp insertions in acute myeloid leukemia (AML). After optimizing multiple parameters, CoHIT achieves a detection limit of 0.01% and rapid results within 30 minutes, without wild-type cross-reactivity. It successfully identifies NPM1 mutations in 30 out of 108 AML patients and demonstrates potential in monitoring minimal residual disease (MRD) through continuous sample analysis from three patients. The CoHIT method is also competent for detecting indels of KIT, BRAF, and EGFR genes. Integration with lateral flow test strips and microfluidic chips highlights CoHIT's adaptability and multiplexing capability, promising significant advancements in clinical cancer diagnostics.","Genetic testing is crucial for precision cancer medicine. Here, the authors develop a one-pot ERA-CRISPR assay to detect variable same-site indels, using an engineered AsCas12a variant with improved mismatch tolerance and broadened PAM scope."]
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收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China (National Science Foundation of China)[2022A1515110852] ; Guangdong Basic and Applied Basic Research Foundation["82300264","81770179","82370176"] ; National Natural Science Foundation of China[2023M742696] ; China Postdoctoral Science Foundation[202102]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001248267400004
出版者	NATURE PORTFOLIO
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/395896
专题	生命科学与技术学院生命科学与技术学院_PI研究组_黄行许组生命科学与技术学院_博士生
通讯作者	Li, Kui; Wang, Xinjie; Zhou, Fuling
作者单位	1.Wuhan Univ, Dept Hematol, Zhongnan Hosp, Wuhan, Peoples R China 2.Wuhan Univ, Shenzhen Res Inst, Shenzhen, Peoples R China 3.Wuhan Univ, Coll Chem & Mol Sci, Wuhan, Peoples R China 4.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Guangdong Lab Lingnan Modeatarn Agr,Genome Anal La, Shenzhen, Peoples R China 5.Wuhan Univ, Dept Radiat & Med Oncol, Zhongnan Hosp, Wuhan, Peoples R China 6.Guangzhou Med Univ, Dept Reprod Med, Affiliated Hosp 3, Guangzhou, Peoples R China 7.Zhejiang Lab, Hangzhou, Peoples R China 8.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yin,Liu, Xinyi,Wei, Dongyi,et al. CoHIT: a one-pot ultrasensitive ERA-CRISPR system for detecting multiple same-site indels[J]. NATURE COMMUNICATIONS,2024,15(1).
APA	Liu, Yin.,Liu, Xinyi.,Wei, Dongyi.,Dang, Lu.,Xu, Xiaoran.,...&Zhou, Fuling.(2024).CoHIT: a one-pot ultrasensitive ERA-CRISPR system for detecting multiple same-site indels.NATURE COMMUNICATIONS,15(1).
MLA	Liu, Yin,et al."CoHIT: a one-pot ultrasensitive ERA-CRISPR system for detecting multiple same-site indels".NATURE COMMUNICATIONS 15.1(2024).