Super-resolution imaging of fast morphological dynamics of neurons in behaving animals

doi:10.1038/s41592-024-02535-9

	Super-resolution imaging of fast morphological dynamics of neurons in behaving animals
	Zhang, Yujie 1,2; Bai, Lu1,2 ; Wang, Xin 1,2; Zhao, Yuchen 1,2; Zhang, Tianlei 1,2; Ye, Lichen 1; Du, Xufei 1,2; Zhang, Zhe 1; Du, Jiulin1,2,3 ; Wang, Kai1,2,3
	2024-11-01
发表期刊	NATURE METHODS (IF:36.1[JCR-2023],45.6[5-Year])
ISSN	1548-7091
EISSN	1548-7105
DOI	10.1038/s41592-024-02535-9
摘要	["Neurons are best studied in their native states in which their functional and morphological dynamics support animals' natural behaviors. Super-resolution microscopy can potentially reveal these dynamics in higher details but has been challenging in behaving animals due to severe motion artifacts. Here we report multiplexed, line-scanning, structured illumination microscopy, which can tolerate motion of up to 50 mu m s-1 while achieving 150-nm and 100-nm lateral resolutions in its linear and nonlinear forms, respectively. We continuously imaged the dynamics of spinules in dendritic spines and axonal boutons volumetrically over thousands of frames and tens of minutes in head-fixed mouse brains during sleep-wake cycles. Super-resolution imaging of axonal boutons revealed spinule dynamics on a scale of seconds. Simultaneous two-color imaging further enabled analyses of the spatial distributions of diverse PSD-95 clusters and opened up possibilities to study their correlations with the structural dynamics of dendrites in the brains of head-fixed awake mice.","A variant of structured illumination microscopy called MLS-SIM allows super-resolution imaging of neuronal structures such as spinules, spines and boutons in awake mice."]
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收录类别	PPRN.PPRN ; SCI
语种	英语
资助项目	STI2030-Major Projects (2021ZD0204503) National Natural Science Foundation of China (32125020, 32321003) Shanghai Committee of Science and Technology, China (22JC1403101)["2021ZD0204503","2021ZD0204502","2022ZD0206100"] ; STI2030-Major Projects["32125020","32321003","82271269","U23A20433"] ; National Natural Science Foundation of China[22JC1403101] ; Shanghai Committee of Science and Technology, China["2018YFA0801000","2018YFA0801001"] ; National Key R&D Program of China["2023M743609","BX20240389"]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:001360969900001
出版者	NATURE PORTFOLIO
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/381328
专题	生命科学与技术学院_特聘教授组_王凯组生命科学与技术学院_特聘教授组_杜久林组
通讯作者	Wang, Kai
作者单位	1.Chinese Acad Sci, Inst Neurosci, CAS Ctr Excellence Brain Sci & Intelligence Techno, Key Lab Brain Cognit & Brain inspired Intelligence, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Yujie,Bai, Lu,Wang, Xin,et al. Super-resolution imaging of fast morphological dynamics of neurons in behaving animals[J]. NATURE METHODS,2024.
APA	Zhang, Yujie.,Bai, Lu.,Wang, Xin.,Zhao, Yuchen.,Zhang, Tianlei.,...&Wang, Kai.(2024).Super-resolution imaging of fast morphological dynamics of neurons in behaving animals.NATURE METHODS.
MLA	Zhang, Yujie,et al."Super-resolution imaging of fast morphological dynamics of neurons in behaving animals".NATURE METHODS (2024).