Transcriptional reactivation of the tRNA<sup>Ser</sup>/tRNA<sup>Tyr</sup> gene cluster in<italic> Arabidopsis thalian</italic>a root tip

doi:10.1101/2023.09.27.559738

	Transcriptional reactivation of the tRNA^Ser/tRNA^Tyr gene cluster in Arabidopsis thaliana root tip
	Hummel, Guillaume 1; Kumari, Priyanka 1; Wang, Long 2; Mai, Yan-Xia 2; Wang, Nan 1; Shala, Negjmedin 1; Kaya, Emir Can 1,4; Wang, Jia-Wei2,3 ; Liu, Chang 1
	2023-09-28
状态	已发表
摘要	Plants retain an exhaustive tRNA gene repertoire in their nuclear genome. How important are these individuals, how exactly plants orchestrate their usage, and for what purposes, is poorly understood. Arabidopsis thaliana chromosome 1 holds a cluster of tandemly repeated tRNA^Ser and tRNA^Tyr genes (SYY cluster). They intersect with constitutive heterochromatin and are transcriptionally silenced. Here, we resolve the tissular expression pattern of this cluster along seedling establishment. We show the root cap columella and few adjacent lateral root cap cells to be the main sources of SYY cluster tRNAs. The transcriptional reactivation of this genomic region occurs there although elevated DNA methylation levels. Furthermore, we evidence that these cells are able to accumulate high levels of a transgenic EXTENSIN rich in serine and tyrosine, and that the CRISPR/Cas9 deletion of the SYY cluster alters such phenomenon. Altogether, our work sheds light on pioneering evidence of a developmental and cell-specific expression program for a plant tRNA gene.
关键词	Transfer RNA tandem repeat chromatin translation root EXTENSIN
语种	英语
DOI	10.1101/2023.09.27.559738
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:85308448
WOS类目	Plant Sciences
资助项目	German Research Foundation["INST 37/935-1 FUGG","757600","32388201","XDB27030101"]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/381277
专题	生命科学与技术学院_特聘教授组_王佳伟组
通讯作者	Hummel, Guillaume; Liu, Chang
作者单位	1.Univ Hohenheim, Inst Biol, Dept Epigenet, Otto Sander Str 5, D-70599 Stuttgart, Baden Wurttemberg, Germany 2.Chinese Acad Sci CAS, Inst Plant Physiol & Ecol SIPPE, CAS Ctr Excellence Mol Plant Sci CEMPS, Natl Key Lab Plant Mol Genet NKLPMG, Shanghai 200032, Peoples R China 3.ShanghaiTech Univ, Shanghai 200031, Peoples R China 4.Nigde Omer Halisdemir Univ, Ayhan Sahenk Fac Agr Sci & Technol, Dept Agr Genet Engn, Merkez Campus, TR-51240 Nigde, Turkiye
推荐引用方式 GB/T 7714	Hummel, Guillaume,Kumari, Priyanka,Wang, Long,et al. Transcriptional reactivation of the tRNA^Ser/tRNA^Tyr gene cluster in Arabidopsis thaliana root tip. 2023.