The robust, high-throughput, and temporally regulated roxCre and loxCre reporting systems for genetic modifications in vivo

doi:10.1101/2024.04.23.590680

	The robust, high-throughput, and temporally regulated roxCre and loxCre reporting systems for genetic modifications in vivo
	Shi, Mengyang 1; Li, Jie 1; Liu, Xiuxiu 1; Liu, Kuo 2; Pu, Wenjuan 1; Weng, Wendong 1; Zhang, Shaohua 1; Zhao, Huan 1; Lui, Kathy O.3,4; Zhou, Bin1,2,5
	2024-04-23
状态	已发表
摘要	Cre-loxP technology, a cornerstone in fate mapping and in vivo gene function studies, faces challenges in achieving precise and efficient conditional mutagenesis through inducible systems. In this study, we introduce two innovative genetic tools designed to overcome these limitations. The first, loxCre, facilitates conditional gene targeting by allowing any CreER line to induce Cre expression with significantly enhanced efficiency. The second, roxCre, enables DreER-mediated Cre release, paving the way for intersectional genetic manipulation that permits both increased precision and efficiency. Both tools incorporate a fluorescent reporter for genetic lineage tracing, revealing efficient gene knockout in cells marked by the reporter simultaneously. These strategies hold great potential for precise and efficient exploration of lineage-specific gene functions, marking a significant advancement in genetic research methodologies.
语种	英语
DOI	10.1101/2024.04.23.590680
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:88600235
WOS类目	Genetics & Heredity
资助项目	National Key Research & Development Program of China["2023YFA1800700","82088101","YSBR-012","JCYJ-SHFY-2021-0","2022ZZ01015"] ; National Natural Science Foundation of China["32370897","B04006C01600515","2022YFA1104200"] ; CAS Project for Young Scientists in Basic Research["2023YFA1801300","32100648"] ; Shanghai Pilot Program for Basic Research-CAS, Shanghai Branch["2020YFA0803202","32370783"] ; Research Funds of Hangzhou Institute for Advanced Study[32100592]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/372897
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_周斌组
通讯作者	Zhou, Bin
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci,New Cornerstone Sci Lab,State Key Lab Cell Biol, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Life Sci, Key Lab Syst Hlth Sci Zhejiang Prov, Hangzhou, Peoples R China 3.Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Hong Kong, Peoples R China 4.Chinese Univ Hong Kong, Prince Wales Hosp, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Shi, Mengyang,Li, Jie,Liu, Xiuxiu,et al. The robust, high-throughput, and temporally regulated roxCre and loxCre reporting systems for genetic modifications in vivo. 2024.