Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists

doi:10.3390/biom14040457

	Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists
	Wang T(王涛)1,2 ; Xixi Chen1,2 ; Guang Yang1 ; Xiaojie Shi1
	2024-04-09
发表期刊	BIOMOLECULES (IF:4.8[JCR-2023],5.4[5-Year])
ISSN	2218-273X
EISSN	2218-273X
卷号	14 期号:4
发表状态	已发表
DOI	10.3390/biom14040457
摘要	There is a high demand for agonist biomolecules such as cytokine surrogates in both biological and medicinal research fields. These are typically sourced through natural ligand engineering or affinity-based screening, followed by individual functional validation. However, efficient screening methods for identifying rare hits within immense libraries are very limited. In this research article, we introduce a phenotypic screening method utilizing biological receptor activation-dependent cell survival (BRADS). This method offers a high-throughput, low-background, and cost-effective approach that can be implemented in virtually any biochemical laboratory setting. As a proof-of-concept, we successfully identified a surrogate for human leptin following a two-week cell culture process, without the need for specialized high-throughput equipment or reagents. This surrogate effectively emulates the activity of native human leptin in cell validation assays. Our findings not only underscore the effectiveness of BRADS but also suggest its potential applicability to a broad range of biological receptors, including Notch and GPCRs.
关键词	phenotypic screening high-throughput screening cytokine surrogate receptor agonist biological receptor activation-dependent cell survival (BRADS)
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:001209939300001
出版者	MDPI
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/364725
专题	生命科学与技术学院_博士生免疫化学研究所免疫化学研究所_特聘教授组_功能筛选实验室
通讯作者	Xiaojie Shi
作者单位	1.Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China 2.School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China
第一作者单位	免疫化学研究所; 生命科学与技术学院
通讯作者单位	免疫化学研究所
第一作者的第一单位	免疫化学研究所
推荐引用方式 GB/T 7714	Wang T,Xixi Chen,Guang Yang,et al. Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists[J]. BIOMOLECULES,2024,14(4).
APA	Wang T,Xixi Chen,Guang Yang,&Xiaojie Shi.(2024).Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists.BIOMOLECULES,14(4).
MLA	Wang T,et al."Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists".BIOMOLECULES 14.4(2024).